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Insights into Trans...
Insights into Translocation Mechanism and Ribosome Evolution from Cryo-EM Structures of Translocation Intermediates of Giardia intestinalis
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Majumdar, Soneya (författare)
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Emmerich, Andrew (författare)
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Krakovka, Sascha (författare)
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visa fler...
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Mandava, Chandra Sekhar (författare)
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Svärd, Staffan (författare)
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Sanyal, Suparna (författare)
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- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
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- Giardia intestinalis is a protozoan parasite that causes diarrhea in humans. Using single-particle cryo-Electron Microscopy, we have determined high-resolution structures of six naturally populated translocation intermediates, from ribosomes isolated directly from actively growing Giardia cells. The highly compact and uniquely GC-rich Giardia ribosomes possess eukaryotic rRNAs and ribosomal-proteins, but retain some bacterial features. The translocation intermediates, with naturally-bound tRNAs and eEF2, display characteristic ribosomal intersubunit rotation and small subunit’s head swiveling - universal for translocation. In addition, we observe the eukaryote-specific ‘subunit rolling’ dynamics, albeit with limited features. Finally, the eEF2•GDP state features a uniquely positioned ‘leaving Pi’ that proposes hitherto unknown molecular events of Pi- and eEF2 release from the ribosome at the final stage of translocation. In summary, our study elucidates the mechanism of translocation in the protists and illustrates evolution of the translation machinery from bacteria to eukaryotes both from the structural and mechanistic perspectives.
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