Sökning: onr:"swepub:oai:DiVA.org:uu-500016" >
Genetically encoded...
Genetically encoded non-canonical amino acids reveal asynchronous dark reversion of chromophore, backbone, and side-chains in EL222
-
- Chaudhari, Aditya S. (författare)
- Czech Acad Sci, Inst Biotechnol, Prague, Czech Republic.;Charles Univ Prague, Fac Sci, Prague, Czech Republic.
-
- Chatterjee, Aditi (författare)
- Czech Acad Sci, Inst Biotechnol, Prague, Czech Republic.;Charles Univ Prague, Fac Sci, Prague, Czech Republic.
-
- Domingos, Catarina A. O. (författare)
- Czech Acad Sci, Inst Biotechnol, Prague, Czech Republic.;Inst Politecn Setubal, Escola Super Tecnol Barreiro, Setubal, Portugal.
-
visa fler...
-
- Andrikopoulos, Prokopis C. (författare)
- Czech Acad Sci, Inst Biotechnol, Prague, Czech Republic.
-
- Liu, Yingliang (författare)
- Czech Acad Sci, Inst Biotechnol, Prague, Czech Republic.
-
- Andersson, Inger (författare)
- Uppsala universitet,Molekylär biofysik,Czech Acad Sci, Inst Biotechnol, Prague, Czech Republic
-
- Schneider, Bohdan (författare)
- Czech Acad Sci, Inst Biotechnol, Prague, Czech Republic.
-
- Lórenz-Fonfría, Víctor A. (författare)
- Univ Valencia, Inst Mol Sci, Paterna, Spain.;Univ Valencia, Inst Mol Sci, Carrer Catedrat Jose Beltran Martinez 2, Paterna 46980, Spain.
-
- Fuertes, Gustavo (författare)
- Czech Acad Sci, Inst Biotechnol, Prague, Czech Republic.;Czech Acad Sci, Inst Biotechnol, Prumyslova 595, Vestec 25250, Czech Republic.
-
visa färre...
-
Czech Acad Sci, Inst Biotechnol, Prague, Czech Republic;Charles Univ Prague, Fac Sci, Prague, Czech Republic. Czech Acad Sci, Inst Biotechnol, Prague, Czech Republic.;Inst Politecn Setubal, Escola Super Tecnol Barreiro, Setubal, Portugal. (creator_code:org_t)
- 2023-03-16
- 2023
- Engelska.
-
Ingår i: Protein Science. - : John Wiley & Sons. - 0961-8368 .- 1469-896X. ; 32:4
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Photoreceptors containing the light-oxygen-voltage (LOV) domain elicit biological responses upon excitation of their flavin mononucleotide (FMN) chromophore by blue light. The mechanism and kinetics of dark-state recovery are not well understood. Here we incorporated the non-canonical amino acid p-cyanophenylalanine (CNF) by genetic code expansion technology at 45 positions of the bacterial transcription factor EL222. Screening of light-induced changes in infrared (IR) absorption frequency, electric field and hydration of the nitrile groups identified residues CNF31 and CNF35 as reporters of monomer/oligomer and caged/decaged equilibria, respectively. Time-resolved multi-probe UV/visible and IR spectroscopy experiments of the lit-to-dark transition revealed four dynamical events. Predominantly, rearrangements around the A'α helix interface (CNF31 and CNF35) precede FMN-cysteinyl adduct scission, folding of α-helices (amide bands), and relaxation of residue CNF151. This study illustrates the importance of characterizing all parts of a protein and suggests a key role for the N-terminal A'α extension of the LOV domain in controlling EL222 photocycle length.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- flavoproteins
- FTIR spectroscopy
- genetic code expansion
- kinetics
- photosensory receptors
- protein structural dynamics
- signal transduction
- site-specific vibrational probes
- time-resolved methods
- UV
- vis spectroscopy
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas