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OptoRheo : Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems
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- Mendonca, Tania (författare)
- Univ Nottingham, Fac Engn, Opt & Photon Res Grp, Nottingham, England.
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- Lis-Slimak, Katarzyna (författare)
- Univ Nottingham, Nottingham Biodiscovery Inst, Sch Med, Nottingham, England.
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- Matheson, Andrew B. (författare)
- Heriot Watt Univ, Inst Biol Chem Biophys & Bioengn, Sch Engn & Phys Sci, Edinburgh, Scotland.
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- Univ Nottingham, Fac Engn, Opt & Photon Res Grp, Nottingham, England Univ Nottingham, Nottingham Biodiscovery Inst, Sch Med, Nottingham, England. (creator_code:org_t)
- Springer Nature, 2023
- 2023
- Engelska.
- Ingår i: Communications Biology. - : Springer Nature. - 2399-3642. ; 6:1
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- A new instrument named OptoRheo combines light sheet fluorescence microscopy and particle tracking microrheology for live imaging and micromechanical sensing of extracellular matrix-cell interactions. Biomechanical cues from the extracellular matrix (ECM) are essential for directing many cellular processes, from normal development and repair, to disease progression. To better understand cell-matrix interactions, we have developed a new instrument named 'OptoRheo' that combines light sheet fluorescence microscopy with particle tracking microrheology. OptoRheo lets us image cells in 3D as they proliferate over several days while simultaneously sensing the mechanical properties of the surrounding extracellular and pericellular matrix at a sub-cellular length scale. OptoRheo can be used in two operational modalities (with and without an optical trap) to extend the dynamic range of microrheology measurements. We corroborated this by characterising the ECM surrounding live breast cancer cells in two distinct culture systems, cell clusters in 3D hydrogels and spheroids in suspension culture. This cutting-edge instrument will transform the exploration of drug transport through complex cell culture matrices and optimise the design of the next-generation of disease models.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
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