Sökning: onr:"swepub:oai:DiVA.org:uu-509059" > The human bone marr...
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000 | 06964naa a2200685 4500 | |
001 | oai:DiVA.org:uu-509059 | |
003 | SwePub | |
008 | 230816s2023 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:153336655 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5090592 URI |
024 | 7 | a https://doi.org/10.1016/j.jaut.2023.1030222 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1533366552 URI |
040 | a (SwePub)uud (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Hensvold, Aaseu Karolinska Institutet,Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.;Stockholm Hlth Reg, Acad Specialist Ctr, Ctr Rheumatol, Stockholm, Sweden.4 aut |
245 | 1 0 | a The human bone marrow plasma cell compartment in rheumatoid arthritis-Clonal relationships and anti-citrulline autoantibody producing cells |
264 | 1 | b Elsevier BV,c 2023 |
338 | a electronic2 rdacarrier | |
520 | a A majority of circulating IgG is produced by plasma cells residing in the bone marrow (BM). Long-lived BM plasma cells constitute our humoral immune memory and are essential for infection-specific immunity. They may also provide a reservoir of potentially pathogenic autoantibodies, including rheumatoid arthritis (RA)-associated anti-citrullinated protein autoantibodies (ACPA). Here we investigated paired human BM plasma cell and peripheral blood (PB) B-cell repertoires in seropositive RA, four ACPA+ RA patients and one ACPA- using two different single-cell approaches, flow cytometry sorting, and transcriptomics, followed by recombinant antibody generation. Immunoglobulin (Ig) analysis of >900 paired heavy-light chains from BM plasma cells identified by either surface CD138 expression or transcriptome profiles (including gene expression of MZB1, JCHAIN and XBP1) demonstrated differences in IgG/A repertoires and N-linked glycosylation between patients. For three patients, we identified clonotypes shared between BM plasma cells and PB memory B cells. Notably, four individuals displayed plasma cells with identical heavy chains but different light chains, which may indicate receptor revision or clonal convergence. ACPA-producing BM plasma cells were identified in two ACPA+ patients. Three of 44 recombinantly expressed monoclonal antibodies from ACPA+ RA BM plasma cells were CCP2+, specifically binding to citrullinated peptides. Out of these, two clones reacted with citrullinated histone-4 and activated neutrophils. In conclusion, single-cell investigation of B-cell repertoires in RA bone marrow provided new understanding of human plasma cells clonal relationships and demonstrated pathogenically relevant disease-associated autoantibody expression in long-lived plasma cells. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Reumatologi och inflammation0 (SwePub)302102 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Rheumatology and Autoimmunity0 (SwePub)302102 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Immunologi inom det medicinska området0 (SwePub)301102 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Immunology in the medical area0 (SwePub)301102 hsv//eng |
653 | a Bone marrow | |
653 | a Plasma cells | |
653 | a Rheumatoid arthritis | |
653 | a Antibody repertoires | |
653 | a Anti-Citrullinated protein autoantibodies  | |
653 | a (ACPA) | |
653 | a Anti-CCP | |
653 | a Receptor revision | |
653 | a Clonal convergence | |
653 | a Ig repertoire | |
700 | 1 | a Horuluoglu, Begumu Karolinska Institutet,Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
700 | 1 | a Sahlstrom, Peteru Karolinska Institutet,Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
700 | 1 | a Thyagarajan, Radhau Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
700 | 1 | a Boada, Juan Sebastian Diazu Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
700 | 1 | a Hansson, Monikau Karolinska Institutet,Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
700 | 1 | a Mathsson Alm, Lindau Uppsala universitet,Institutionen för immunologi, genetik och patologi,Thermo Fisher Sci, Uppsala, Sweden.4 aut0 (Swepub:uu)limat990 |
700 | 1 | a Gerstner, Christinau Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
700 | 1 | a Sippl, Natalieu Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
700 | 1 | a Israelsson, Lenau Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
700 | 1 | a Wedin, Rikardu Karolinska Institutet,Karolinska Univ Hosp, Karolinska Inst, Dept Trauma & Reparat Med, Stockholm, Sweden.;Karolinska Univ Hosp, Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden.4 aut |
700 | 1 | a Steen, Johannau Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
700 | 1 | a Klareskog, Larsu Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
700 | 1 | a Rethi, Benceu Karolinska Institutet4 aut |
700 | 1 | a Catrina, Anca I.u Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.;Stockholm Hlth Reg, Acad Specialist Ctr, Ctr Rheumatol, Stockholm, Sweden.4 aut |
700 | 1 | a Diaz-Gallo, Lina -Marcelau Karolinska Institutet,Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
700 | 1 | a Malmstrom, Vivianneu Karolinska Institutet,Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
700 | 1 | a Gronwall, Carolineu Karolinska Institutet,Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.4 aut |
710 | 2 | a Karolinska Institutetb Karolinska Univ Hosp, Karolinska Inst, Div Rheumatol, Dept Med,Ctr Mol Med, Stockholm, Sweden.;Stockholm Hlth Reg, Acad Specialist Ctr, Ctr Rheumatol, Stockholm, Sweden.4 org |
773 | 0 | t Journal of Autoimmunityd : Elsevier BVg 136q 136x 0896-8411x 1095-9157 |
856 | 4 | u https://doi.org/10.1016/j.jaut.2023.103022y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1788447/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-509059 |
856 | 4 8 | u https://doi.org/10.1016/j.jaut.2023.103022 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:153336655 |
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