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Microfluidic system...
Microfluidic system with integrated nanocellulose cell culture substrate to study alignment of human umbilical vein endothelial cells in relation to external physical cues
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- Wu, Lulu (författare)
- Uppsala universitet,Nanoteknologi och funktionella material,Science for Life Laboratory, SciLifeLab
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- Atif, Abdul Raouf, 1996- (författare)
- Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab
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- Agnihotri, Sagar N. (författare)
- Uppsala universitet,Institutionen för materialvetenskap,Science for Life Laboratory, SciLifeLab
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visa fler...
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- Barbe, Laurent (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Mikrosystemteknik
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- Porras Hernández, Ana Maria (författare)
- Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab
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- Ferraz, Natalia, 1976- (författare)
- Uppsala universitet,Nanoteknologi och funktionella material
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- Tenje, Maria (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för materialvetenskap
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visa färre...
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- In this work, aligned cationic cellulose nanofibrils (c-CNF) were integrated into a microfluidic channel to guide cell attachment of human umbilical vein endothelial cells (HUVECs) and the effect of different mechanical cues on cell orientation was investigated. The on-chip cultured cells were exposed to external stimuli by the c-CNF topography and a fluid flow-induced shear stress, either separately or combined. Fluorescent images of the c-CNF pattern stained with calcofluor white and HUVECs stained for F-actin fibers and cell nuclei were obtained and used to quantify orientation of the CNFs, the F-actin fibers and the cell nuclei together with the eccentricity of the nuclei. Compared to the control, where the cells were cultured on a smooth surface in static conditions, cells cultured on the c-CNF pattern alone showed a clear alignment to the underlying microtopography. Cells cultured on a smooth surface responded slightly to the external mechanical stimuli indicating that the cell orientation was more strongly affected by c-CNF topography than the shear stress. With these results, we established a platform that can de-couple external mechanical stimuli originating from surface topography and shear stress to increase our understanding of how cells react to these factors when cultured in microfluidic in vitro systems.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
Nyckelord
- cellulose nanofibrils
- microfluidics
- cellular orientation
- shear stress
- topography
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)