Sökning: onr:"swepub:oai:DiVA.org:uu-51578" >
Differential activa...
Differential activation and regulation of mitogen activated protein kinases through the antigen receptor and CD40 in human B cells
-
Sakata, Naoki (författare)
-
Kawasome, Hideki (författare)
-
Terada, Naohiro (författare)
-
visa fler...
-
- Gerwins, Pär (författare)
- Uppsala universitet,Institutionen för genetik och patologi,Institutionen för medicinsk biokemi och mikrobiologi
-
Johnson, Gary L. (författare)
-
Gelfand, Erwin W. (författare)
-
visa färre...
-
(creator_code:org_t)
- 1999
- 1999
- Engelska.
-
Ingår i: European Journal of Immunology. - 0014-2980 .- 1521-4141. ; 29:9, s. 2999-3008
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- In human B cells, antigen receptor ligation and CD40 ligation are known to activate the extracellular-regulated kinases (ERK) and c-Jun N-terminal kinase (JNK) pathways, which in turn regulate many important B cell functions. We previously reported that antigen receptor ligation activated the ERK pathway whereas CD40 ligation activated the JNK/stress-activated protein kinase (SAPK) pathway. Here, we demonstrate that another SAPK, p38/Hog1, is activated by both antigen receptor ligation or CD40 ligation in a human B-lymphoblastoid cell line and tonsillar B cells. Wortmannin, an inhibitor of phosphatidylinositol 3-kinase, partially inhibited ERK2 and p38 activation triggered through the B cell receptor whereas activation of JNK1 and p38 through CD40 was not affected. PD98059, a specific inhibitor of mitogen-activated extracellular response kinase kinase (MEK), significantly inhibited ERK2 activation and partially inhibited p38 activation triggered by anti-IgM antibody treatment, but did not affect CD40-dependent signaling events. In addition, anti-IgM antibody-induced signaling pathways were shown to be PKC-dependent in contrast to the CD40-induced signaling pathways. Thus, the B cell receptor and CD40 recruit the ERK, JNK and p38 pathways by using different upstream effectors.
Nyckelord
- B cell receptor
- Activation
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas