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A novel serum calpr...
A novel serum calprotectin (MRP8/14) particle-enhanced immuno-turbidimetric assay (sCAL turbo) helps to differentiate systemic juvenile idiopathic arthritis from other diseases in routine clinical laboratory settings
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- Foell, Dirk (författare)
- Univ Childrens Hosp Muenster, Dept Pediat Rheumatol & Immunol, D-48149 Munster, Germany.
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- Saers, Melanie (författare)
- Univ Childrens Hosp Muenster, Dept Pediat Rheumatol & Immunol, D-48149 Munster, Germany.
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- Park, Carolin (författare)
- Univ Childrens Hosp Muenster, Dept Pediat Rheumatol & Immunol, D-48149 Munster, Germany.
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- Brix, Ninna (författare)
- Aalborg Univ Hosp, Dept Pediat & Adolescent Med, Aalborg, Denmark.
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- Glerup, Mia (författare)
- Aarhus Univ Hosp, Dept Pediat & Adolescent Med, Aarhus, Denmark.
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- Kessel, Christoph (författare)
- Univ Childrens Hosp Muenster, Dept Pediat Rheumatol & Immunol, D-48149 Munster, Germany.
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- Wittkowski, Helmut (författare)
- Univ Childrens Hosp Muenster, Dept Pediat Rheumatol & Immunol, D-48149 Munster, Germany.
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- Hinze, Claas (författare)
- Univ Childrens Hosp Muenster, Dept Pediat Rheumatol & Immunol, D-48149 Munster, Germany.
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- Berntson, Lillemor, 1957- (författare)
- Uppsala universitet,Pediatrisk inflammations- och metabolismforskning samt barnhälsa
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- Fasth, Anders, 1945 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics
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- Myrup, Charlotte (författare)
- Copenhagen Univ Hosp, Dept Pediat, Copenhagen, Denmark.
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- Nordal, Ellen (författare)
- Univ Hosp North Norway, Dept Pediat, Tromso, Norway.;Arctic Univ Norway, Dept Clin Med, Tromso, Norway.
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- Rygg, Marite (författare)
- St Olavs Hosp, Dept Pediat, Trondheim, Norway.;Norwegian Univ Sci & Technol, Dept Clin & Mol Med, Trondheim, Norway.
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- Hasle, Henrik (författare)
- Aarhus Univ Hosp, Dept Pediat & Adolescent Med, Aarhus, Denmark.
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- Albertsen, Birgitte Klug (författare)
- Aarhus Univ Hosp, Dept Pediat & Adolescent Med, Aarhus, Denmark.
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- Herlin, Troels (författare)
- Aarhus Univ Hosp, Dept Pediat & Adolescent Med, Aarhus, Denmark.
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- Holzinger, Dirk (författare)
- Univ Duisburg Essen, Dept Pediat Hematol Oncol, Essen, Germany.;Bochum Univ Appl Hlth Sci, Dept Appl Hlth Sci, Bochum, Germany.
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- Niederberger, Christian (författare)
- BUHLMANN Labs AG, Schonenbuch, Switzerland.
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- Schlüter, Bernhard (författare)
- Univ Hosp Muenster, Cent Lab, Munster, Germany.
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Univ Childrens Hosp Muenster, Dept Pediat Rheumatol & Immunol, D-48149 Munster, Germany Aalborg Univ Hosp, Dept Pediat & Adolescent Med, Aalborg, Denmark. (creator_code:org_t)
- Springer, 2023
- 2023
- Engelska.
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Ingår i: Molecular and Cellular Pediatrics. - : Springer. - 2194-7791. ; 10
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://gup.ub.gu.se...
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Abstract
Ämnesord
Stäng
- Background: Differential diagnosis in children with signs of unprovoked inflammation can be challenging. In particular, differentiating systemic juvenile idiopathic arthritis (SJIA) from other diagnoses is difficult. We have recently validated the complex of myeloid-related proteins 8/14 (MRP8/14, also known as S100A8/A9 complex or serum calprotectin) as a helpful biomarker supporting the diagnosis of SJIA. The results were subsequently confirmed with a commercial ELISA. However, further optimization of the analytical technology is important to ensure its feasibility for large-scale use in routine laboratory settings.Methods: To evaluate the accuracy in identifying children with SJIA, the performance of a particle-enhanced immuno-turbidimetric assay for serum calprotectin (sCAL turbo) on an automated laboratory instrument was analyzed. Samples from 615 children were available with the diagnoses SJIA (n = 99), non-systemic JIA (n = 169), infections (n = 51), other inflammatory diseases (n = 126), and acute lymphoblastic leukemia (ALL, n = 147). In addition, samples from 23 healthy controls were included.Results: The sCAL turbo assay correlated well with the MRP8/14 ELISA used in previous validation studies (r = 0.99, p < 0.001). It could reliably differentiate SJIA from all other diagnoses with significant accuracy (cutoff at 10,500 ng/ml, sensitivity 84%, specificity 94%, ROC area under curve 0.960, p < 0.001).Conclusions: Serum calprotectin analyses are a helpful tool supporting the diagnosis of SJIA in children with prolonged fever or inflammatory disease. Here, we show that an immuno-turbidimetric assay for detection of serum calprotectin on an automated laboratory instrument can be implemented in clinical laboratory settings to facilitate its use as a diagnostic routine test in clinical practice.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Pediatrik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Pediatrics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
Nyckelord
- Biomarkers
- Fever of unknown origin
- Still's syndrome
- Systemic inflammation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Foell, Dirk
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Saers, Melanie
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Park, Carolin
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Brix, Ninna
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Glerup, Mia
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Kessel, Christop ...
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Wittkowski, Helm ...
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Hinze, Claas
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Berntson, Lillem ...
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Fasth, Anders, 1 ...
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Myrup, Charlotte
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Nordal, Ellen
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Rygg, Marite
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Hasle, Henrik
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Albertsen, Birgi ...
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Herlin, Troels
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Holzinger, Dirk
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Niederberger, Ch ...
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Schlüter, Bernha ...
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Molecular and Ce ...
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Uppsala universitet
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Göteborgs universitet