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Hgf/sf induces meso...
Hgf/sf induces mesothelial cell migration and proliferation by autocrineand paracrine pathways.
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Warn, Richard (författare)
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Harvey, Pascale (författare)
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Warn, Alba (författare)
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Foley-Comer, Adam (författare)
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- Heldin, Paraskevi (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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Versnel, Marjan (författare)
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Arakaki, Naokatu (författare)
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Daikuhara, Yasushi (författare)
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Laurent, Geoffrey J. (författare)
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Herrick, Sarah E. (författare)
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Mutsaers, Steven E. (författare)
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(creator_code:org_t)
- 2001
- 2001
- Engelska.
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Ingår i: Exp Cell Res. ; 267, s. 258-
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Mesothelial repair differs from that of other epithelial-like surfaces as healing does not occur solely by centripetal in-growth of cells as a sheet from the wound margins. Mesothelial cells lose their cell-cell junctions, divide, and adopt a fibroblast-like morphology while scattering across and covering the wound surface. These features are consistent with a cellular response to hepatocyte growth factor/scatter factor (HGF/SF). In this study, we examined the ability of mesothelial cells to secrete HGF/SF and investigated its possible role as an autocrine regulator of mesothelial cell motility and proliferation. We found that human primary mesothelial cells expressed HGF/SF mRNA and secreted active HGF/SF into conditioned medium as determined by ELISA and in a scattering bioassay. These cells also expressed the HGF/SF receptor, Met, as shown by RT-PCR and by Western blot analysis and immunofluorescence. Incubation of mesothelial cells with neutralizing antibodies to HGF/SF decreased cell migration to 25% of controls, whereas addition of HGF/SF disrupted cell-cell junctions and induced scattering and enhanced mesothelial cell migration. Furthermore, HGF/SF showed a small but significant mitogenic effect on all mesothelial cell lines examined. In conclusion, HGF/SF is produced by mesothelial cells and induces both motility and proliferation of these cells. These data are consistent with HGF/SF playing an autocrine role in mesothelial healing.
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Warn, Richard
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Harvey, Pascale
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Warn, Alba
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Foley-Comer, Ada ...
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Heldin, Paraskev ...
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Versnel, Marjan
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visa fler...
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Arakaki, Naokatu
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Daikuhara, Yasus ...
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Laurent, Geoffre ...
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Herrick, Sarah E ...
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Mutsaers, Steven ...
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visa färre...
- Artiklar i publikationen
- Exp Cell Res
- Av lärosätet
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Uppsala universitet