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Characterization of...
Characterization of a Novel Sulfated Carbohydrate Unit Implicated in the CarbohydrateCarbohydrate-mediated Cell Aggregation of the Marine Sponge Microciona prolifera
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- Spillmann, Dorothe (författare)
- University of Turku
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Thomas-Oates, J.E. (författare)
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van Kuik, A (författare)
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Vliegenhart, J.F.G. (författare)
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Misevic, G (författare)
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Burger, M.M. (författare)
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Finne, J (författare)
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visa färre...
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(creator_code:org_t)
- 1995
- 1995
- Engelska.
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Ingår i: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 270, s. 5089-5097
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Species-specific cell reaggregation in the marine sponge Microciona prolifera is mediated by an adhesion proteoglycan. Two interactions are involved in the process: a Ca(2+)-dependent homophilic binding between proteoglycan molecules and a Ca(2+)-independent binding between the molecule and cells. Both interactions are mediated by the glycan moieties of the proteoglycan. The interaction of the proteoglycan with itself has been characterized as a carbohydrate-carbohydrate interaction of multiple low affinity sites. The monoclonal antibodies Block 1 and Block 2 raised against the purified aggregation proteoglycan and selected for inhibition of aggregation bind to these glycans. In a previous report the structure, [formula: see text] was assigned to the oligosaccharide reacting with Block 1 antibody (Spillmann, D., Hård, K., Thomas-Oates, J., Vliegenthart, J. F. G., Misevic, G., Burger, M. M., and Finne, J. (1993) J. Biol. Chem. 268, 13378-13387). By the technique of attaching the water-soluble acid-degraded fragments to a lipid carrier for immunochemical detection and by chemical, enzymatic and spectroscopic methods the structure, [formula: see text] was assigned to the oligosaccharide reacting with the aggregation-blocking monoclonal antibody Block 2. The structure, [formula: see text] was assigned to a major nonreactive oligosaccharide, which outlined the molecular requirements of antibody binding of the two aggregation-associated epitopes. These data demonstrate that two different functional sites with distinct structural characteristics and antibody reactivities are involved in the reaggregation of sponge cells, a model of carbohydrate-carbohydrate-mediated cell interactions.
Nyckelord
- MEDICINE
- MEDICIN
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- art (ämneskategori)
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