Search: WFRF:(Varghese Oommen P. 1977 ) >
Fine-tuning Dynamic...
Fine-tuning Dynamic Cross-linking for Enhanced 3D Bioprinting of Hyaluronic Acid Hydrogels
-
- Tavakoli, Shima (author)
- Uppsala universitet,Makromolekylär kemi
-
- Krishnan, Nithiyanandan (author)
- Uppsala universitet,Makromolekylär kemi
-
- Mokhtari, Hamidreza (author)
- Uppsala universitet,Makromolekylär kemi
-
show more...
-
- Oommen, Oommen P. (author)
- Tampere Univ, Fac Med & Hlth Technol, Bioengn & Nanomed Grp, Tampere 33720, Finland.;BioMediTech Inst, Tampere 33720, Finland.
-
- Varghese, Oommen P., 1977- (author)
- Uppsala universitet,Makromolekylär kemi
-
show less...
-
(creator_code:org_t)
- Wiley-VCH Verlagsgesellschaft, 2024
- 2024
- English.
-
In: Advanced Functional Materials. - : Wiley-VCH Verlagsgesellschaft. - 1616-301X .- 1616-3028. ; 34:4
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- 3D bioprinting of stem cells shows promise for medical applications, but the development of an efficient bioink remains a challenge. Recently, the emergence of dynamically cross-linked hydrogels has advanced this field to obtain self-healing materials. However, more advanced bioinks are needed that display optimum gelling kinetics, viscoelasticity, shear-thinning property, structural fidelity, and hold the printed structures sufficiently long enough that allow maturation of the new tissue. Here, a novel extracellular matrix-based bioink for human mesenchymal stem cells (hMSCs) is presented. Hyaluronic acid (HA) is modified with cysteine and aldehyde functional groups, creating hydrogels with dual cross-linking of disulfide and thiazolidine products. The investigation demonstrates that this cross-linking significantly improves hydrogel stability and biological properties. The bioink exhibits fast gelation kinetics, shear-thinning, shape-maintaining properties, high cell survival after printing with >2-fold increase in stemness marker (OCT3/4 and NANOG), and supports cell proliferation and migration. Disulfide cross-linking contributes to self-healing and cell migration, while thiazolidine cross-linking reduces gelation time, enhances long-term stability, and supports cell proliferation. Overall, the HA-based bioink fulfills the requirements for successful 3D printing of stem cells, providing a promising solution for cell therapy and regenerative medicine.
Subject headings
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- 3D bioprinting
- disulfides
- hyaluronic acids
- hydrogels
- thiazolidine
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database