Sökning: onr:"swepub:oai:DiVA.org:uu-528373" > Anchoring the ethyl...
Fältnamn | Indikatorer | Metadata |
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000 | 03141naa a2200409 4500 | |
001 | oai:DiVA.org:uu-528373 | |
003 | SwePub | |
008 | 240521s2022 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5283732 URI |
024 | 7 | a https://doi.org/10.1016/j.biteb.2022.1011782 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Gao, Xiangu Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China.4 aut |
245 | 1 0 | a Anchoring the ethylene-forming enzyme to photosystem-related proteins to improve ethylene production in engineered Synechocystis PCC 6803 |
264 | 1 | b Elsevier,c 2022 |
338 | a electronic2 rdacarrier | |
500 | a De två första författarna delar förstaförfattarskapet | |
520 | a Ethylene production in engineered cyanobacteria offers a sustainable alternative to the traditional production from fossil resources. Protein fusions and anchoring strategies are promising routes to augment the stability of recombinant proteins. Here we attempted to anchor the ethylene-forming enzyme (Efe) to several photosystemrelated proteins (including D1, PetF, and PsaE) in order to improve the ethylene producing capability in engineered cells of Synechocystis PCC 6803. Generated strains harboring fused Efe with D1 or PetF showed respectively 1.6- and 1.5-fold increase in ethylene production compared to a control strain harboring only Efe. Additionally, strains co-expressing a non-fused Efe with PsaE or PetF showed respectively 3.5- and 3.1-fold increase in ethylene production compared to the control strain. This work demonstrates the feasibility to incorporate (anchor or co-express) target enzyme(s) with specific photosystem components for facilitating highly efficient production of bulk chemicals in engineered cyanobacteria. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
653 | a Ethylene | |
653 | a Metabolic engineering | |
653 | a Protein anchoring | |
653 | a Photosystem | |
653 | a cyanobacteria | |
700 | 1 | a Liu, Xufengu Uppsala universitet,Molekylär biomimetik,Mikrobiell kemi4 aut0 (Swepub:uu)xufli265 |
700 | 1 | a Jing, Xinu Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China.4 aut |
700 | 1 | a Lindblad, Peteru Uppsala universitet,Molekylär biomimetik,Mikrobiell kemi4 aut0 (Swepub:uu)peterlb |
710 | 2 | a Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China.b Molekylär biomimetik4 org |
773 | 0 | t Bioresource Technology Reportsd : Elsevierg 19q 19x 2589-014X |
856 | 4 | u https://doi.org/10.1016/j.biteb.2022.101178y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1859290/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-528373 |
856 | 4 8 | u https://doi.org/10.1016/j.biteb.2022.101178 |
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