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Removal of hypersig...
Removal of hypersignaling endosomes by simaphagy
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- Migliano, Simona M. (författare)
- Univ Oslo, Fac Med, Ctr Canc Cell Reprogramming, Oslo, Norway.;Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway.
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- Schultz, Sebastian W. (författare)
- Univ Oslo, Fac Med, Ctr Canc Cell Reprogramming, Oslo, Norway.;Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway.
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- Wenzel, Eva M. (författare)
- Univ Oslo, Fac Med, Ctr Canc Cell Reprogramming, Oslo, Norway.;Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway.
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- Takáts, Szabolcs (författare)
- Univ Oslo, Fac Med, Ctr Canc Cell Reprogramming, Oslo, Norway.;Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway.;Eotvos Lorand Univ, Dept Anat Cell & Dev Biol, Budapest, Hungary.
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- Liu, Dan (författare)
- Univ Oslo, Fac Med, Ctr Canc Cell Reprogramming, Oslo, Norway.;Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway.
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- Mørk, Silje (författare)
- Univ Oslo, Fac Med, Ctr Canc Cell Reprogramming, Oslo, Norway.;Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway.
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- Tan, Kia Wee (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi,Univ Oslo, Fac Med, Ctr Canc Cell Reprogramming, Oslo, Norway; Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway
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- Rusten, Tor Erik (författare)
- Univ Oslo, Fac Med, Ctr Canc Cell Reprogramming, Oslo, Norway.;Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway.
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- Raiborg, Camilla (författare)
- Univ Oslo, Fac Med, Ctr Canc Cell Reprogramming, Oslo, Norway.;Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway.
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- Stenmark, Harald (författare)
- Univ Oslo, Fac Med, Ctr Canc Cell Reprogramming, Oslo, Norway.;Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway.
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Univ Oslo, Fac Med, Ctr Canc Cell Reprogramming, Oslo, Norway;Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway. Univ Oslo, Fac Med, Ctr Canc Cell Reprogramming, Oslo, Norway.;Oslo Univ Hosp, Inst Canc Res, Dept Mol Cell Biol, Oslo, Norway.;Eotvos Lorand Univ, Dept Anat Cell & Dev Biol, Budapest, Hungary. (creator_code:org_t)
- Taylor & Francis, 2024
- 2024
- Engelska.
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Ingår i: Autophagy. - : Taylor & Francis. - 1554-8627 .- 1554-8635. ; 20:4, s. 769-791
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Activated transmembrane receptors continue to signal following endocytosis and are only silenced upon ESCRT-mediated internalization of the receptors into intralumenal vesicles (ILVs) of the endosomes. Accordingly, endosomes with dysfunctional receptor internalization into ILVs can cause sustained receptor signaling which has been implicated in cancer progression. Here, we describe a surveillance mechanism that allows cells to detect and clear physically intact endosomes with aberrant receptor accumulation and elevated signaling. Proximity biotinylation and proteomics analyses of ESCRT-0 defective endosomes revealed a strong enrichment of the ubiquitin-binding macroautophagy/autophagy receptors SQSTM1 and NBR1, a phenotype that was confirmed in cell culture and fly tissue. Live cell microscopy demonstrated that loss of the ESCRT-0 subunit HGS/HRS or the ESCRT-I subunit VPS37 led to high levels of ubiquitinated and phosphorylated receptors on endosomes. This was accompanied by dynamic recruitment of NBR1 and SQSTM1 as well as proteins involved in autophagy initiation and autophagosome biogenesis. Light microscopy and electron tomography revealed that endosomes with intact limiting membrane, but aberrant receptor downregulation were engulfed by phagophores. Inhibition of autophagy caused increased intra- and intercellular signaling and directed cell migration. We conclude that dysfunctional endosomes are surveyed and cleared by an autophagic process, simaphagy, which serves as a failsafe mechanism in signal termination.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Autophagy
- endosome
- ESCRT
- receptor degradation
- signaling
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Migliano, Simona ...
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Schultz, Sebasti ...
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Wenzel, Eva M.
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Takáts, Szabolcs
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Liu, Dan
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Mørk, Silje
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visa fler...
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Tan, Kia Wee
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Rusten, Tor Erik
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Raiborg, Camilla
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Stenmark, Harald
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Cellbiologi
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
- Artiklar i publikationen
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Autophagy
- Av lärosätet
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Uppsala universitet