Sökning: onr:"swepub:oai:DiVA.org:uu-532536" >
Differential requir...
Differential requirement for RecFOR pathway components in Thermus thermophilus
-
- Gómez-Campo, Cristina L. (författare)
- Univ Politecn Madrid, Ctr Plant Biotechnol & Genom CBGP, Madrid, Spain.
-
- Abdelmoteleb, Ali (författare)
- Autonomous Univ Madrid, Ctr Mol Biol Severo Ochoa CBM, Higher Council Sci Res CSIC, Dept Mol Biol,Sci Fac, Madrid 28049, Spain.;Menoufia Univ, Fac Agr, Dept Bot, Shibin Al Kawm, Egypt.
-
- Pulido, Verónica (författare)
- Autonomous Univ Madrid, Ctr Mol Biol Severo Ochoa CBM, Higher Council Sci Res CSIC, Dept Mol Biol,Sci Fac, Madrid 28049, Spain.
-
visa fler...
-
- Gost, Marc (författare)
- Autonomous Univ Madrid, Ctr Mol Biol Severo Ochoa CBM, Higher Council Sci Res CSIC, Dept Mol Biol,Sci Fac, Madrid 28049, Spain.
-
- Sánchez-Hevia, Dione L. (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Berenguer, José (författare)
- Autonomous Univ Madrid, Ctr Mol Biol Severo Ochoa CBM, Higher Council Sci Res CSIC, Dept Mol Biol,Sci Fac, Madrid 28049, Spain.
-
- Mencía, Mario (författare)
- Autonomous Univ Madrid, Ctr Mol Biol Severo Ochoa CBM, Higher Council Sci Res CSIC, Dept Mol Biol,Sci Fac, Madrid 28049, Spain.
-
visa färre...
-
Univ Politecn Madrid, Ctr Plant Biotechnol & Genom CBGP, Madrid, Spain Autonomous Univ Madrid, Ctr Mol Biol Severo Ochoa CBM, Higher Council Sci Res CSIC, Dept Mol Biol,Sci Fac, Madrid 28049, Spain.;Menoufia Univ, Fac Agr, Dept Bot, Shibin Al Kawm, Egypt. (creator_code:org_t)
- John Wiley & Sons, 2024
- 2024
- Engelska.
-
Ingår i: Environmental Microbiology Reports. - : John Wiley & Sons. - 1758-2229. ; 16:3
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Recombinational repair is an important mechanism that allows DNA replication to overcome damaged templates, so the DNA is duplicated timely and correctly. The RecFOR pathway is one of the common ways to load RecA, while the RuvABC complex operates in the resolution of DNA intermediates. We have generated deletions of recO, recR and ruvB genes in Thermus thermophilus, while a recF null mutant could not be obtained. The recO deletion was in all cases accompanied by spontaneous loss of function mutations in addA or addB genes, which encode a helicase-exonuclease also key for recombination. The mutants were moderately affected in viability and chromosome segregation. When we generated these mutations in a Δppol/addAB strain, we observed that the transformation efficiency was maintained at the typical level of Δppol/addAB, which is 100-fold higher than that of the wild type. Most mutants showed increased filamentation phenotypes, especially ruvB, which also had DNA repair defects. These results suggest that in T. thermophilus (i) the components of the RecFOR pathway have differential roles, (ii) there is an epistatic relationship of the AddAB complex over the RecFOR pathway and (iii) that neither of the two pathways or their combination is strictly required for viability although they are necessary for normal DNA repair and chromosome segregation.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas