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Epitope mapping of ...
Epitope mapping of a monoclonal antibody that blocks the binding of retinol-binding protein to its receptor
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- Melhus, Håkan (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper,osteo
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Båvik, Claes-Olov (författare)
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- Rask, Lars (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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Peterson, Per A. (författare)
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- Eriksson, Ulf (författare)
- Karolinska Institutet,Uppsala universitet,Institutionen för medicinsk cellbiologi
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(creator_code:org_t)
- Elsevier BV, 1995
- 1995
- Engelska.
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 210:1, s. 105-112
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- To define the receptor binding site of retinol-binding protein (RBP) we have generated monoclonal antibodies (mAbs) to human RBP and examined their ability to interfere with the receptor binding. MAbs to two conserved regions efficiently blocked the binding. No major conformational changes in the protein occurred upon mAb binding, since the mAbs could co-immunoprecipitate the RBP-transthyretin (TTR) complex. One blocking mAb showed reactivity to a synthetic peptide corresponding to one entrance loop of the retinol-binding pocket (amino acid residues 60-70). Thus, our results show that at least one of the entrance loops of the barrel of RBP is located in or close to the receptor binding site. It can also be concluded that the receptor and TTR binding sites involve different regions of RBP.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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