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Small, novel protei...
Small, novel proteins from the mistletoe Phoradendron tomentosum exhibit highly selective cytotoxicity to human breast cancer cells
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- Johansson, S. (författare)
- Uppsala universitet,Avdelningen för farmakognosi
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- Gullbo, Joachim (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper,Cancer Pharmacology and Informatics
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- Lindholm, P. (författare)
- Uppsala universitet,Avdelningen för farmakognosi
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Ek, B. (författare)
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Thunberg, E. (författare)
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- Samuelsson, Gunnar (författare)
- Uppsala universitet,Avdelningen för farmakognosi
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- Larsson, Rolf (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper,Cancer Pharmacology and Informatics
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- Bohlin, Lars (författare)
- Uppsala universitet,Avdelningen för farmakognosi
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- Claeson, Per (författare)
- Uppsala universitet,Avdelningen för farmakognosi
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(creator_code:org_t)
- Springer Science and Business Media LLC, 2003
- 2003
- Engelska.
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Ingår i: Cellular and Molecular Life Sciences (CMLS). - : Springer Science and Business Media LLC. - 1420-682X .- 1420-9071. ; 60:1, s. 165-175
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Four novel proteins (phoratoxins C-F) have been isolated from the North American mistletoe Phoradendron tomentosum. The amino acid sequences of these phoratoxins were determined unambiguously using a combination of Edman degradation and trypsin enzymatic digestion, and by electrospray ionization tandem mass spectrometry sequencing. Phoratoxins C, E and F consist of 46 amino acid residues; and phoratoxin D of 41. All proteins had six cysteines, similar to the earlier described phoratoxins A and B, which are thionins. The cytotoxicity of each protein was evaluated in a human cell line panel that represented several cytotoxic drug-resistance mechanisms. For the half-maximal inhibitory concentrations (IC50 values) of the different cell lines in the panel, correlation with those of standard drugs was low. The most potent cytotoxic phoratoxin C was further tested on primary cultures of human tumor cells from patients. The solid tumor samples from breast cancer cells were 18 times more sensitive to phoratoxin C than the tested hematological tumor samples.
Nyckelord
- Breast cancer
- cytotoxicity drug screening
- mistletoe proteins
- Phoradendron tomentosum
- phoratoxins
- thionins
- tumor cell lines
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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