Sökning: onr:"swepub:oai:DiVA.org:uu-73074" >
RICH-1 has a BIN/Am...
RICH-1 has a BIN/Amphiphysin/Rvsp domain responsible for binding to membrane lipids and tubulation of liposomes
-
- Richnau, Ninna (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
-
- Fransson, Åsa (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
-
Farsad, Khashayar (författare)
-
visa fler...
-
- Aspenström, Pontus (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2004
- 2004
- Engelska.
-
Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 320:3, s. 1034-1042
- Relaterad länk:
-
http://www.ncbi.nlm....
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- RhoGAP interacting with CIP4 homologs-1 (RICH-1) was previously found in a yeast two-hybrid screen for proteins interacting with the SH3 domain of the Cdc42-interacting protein 4 (CIP4). RICH-1 was shown to be a RhoGAP for Cdc42 and Rac. In this study, we show that the BIN/Amphiphysin/Rvsp (BAR) domain in RICH-1 confers binding to membrane lipids, and has the potential to deform spherical liposomes into tubes. In accordance with previous findings for the BAR domains in endophilin and amphiphysin, RICH-1-induced tubes appeared striated. We propose that these striated structures are formed by oligomerization of RICH-1 through a putative coiled-coil region within the BAR domain. In support of this notion, we show that RICH-1 forms oligomers in the presence of the chemical cross-linker BS3. These results point to an involvement of RICH-1 in membrane deformation events.
Nyckelord
- Amino Acid Sequence
- Animals
- Binding Sites
- COS Cells
- Cell Membrane/*metabolism/*ultrastructure
- Cells; Cultured
- Cercopithecus aethiops
- Endothelial Cells/*metabolism
- GTPase-Activating Proteins/*chemistry/*metabolism
- Liposomes/*metabolism
- Membrane Fluidity/*physiology
- Membrane Lipids/*metabolism
- Molecular Sequence Data
- Nerve Tissue Proteins/chemistry/metabolism
- Protein Binding
- Protein Structure; Tertiary
- Research Support; Non-U.S. Gov't
- Research Support; U.S. Gov't; P.H.S.
- Sequence Homology; Amino Acid
- Structure-Activity Relationship
- Swine
- Tissue Distribution
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas