onr:"swepub:oai:DiVA.org:uu-74328"
Sökning: onr:"swepub:oai:DiVA.org:uu-74328" > Human eosinophil pe...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02874naa a2200445 4500 | |
| 001 | oai:DiVA.org:uu-74328 | |
| 003 | SwePub | |
| 008 | 050921s1985 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-743282 URI |
| 040 | a (SwePub)uu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Carlson, Mu Uppsala universitet,Institutionen för medicinska vetenskaper4 aut0 (Swepub:uu)maricarl |
| 245 | 1 0 | a Human eosinophil peroxidase :b purification and characterization |
| 264 | 1 | c 1985 |
| 338 | a print2 rdacarrier | |
| 520 | a Human eosinophil peroxidase (EPO) was isolated from granules from granulocytes of a patient with hypereosinophilia. The granules were extracted by means of 0.2 M NaAc, pH 4.0. The purification steps included gel filtration chromatography on Sephadex G-75 superfine and ion-exchange chromatography on CM-Sephadex G-50. The purified protein showed one band on agarose-electrophoresis, a high peroxidase activity, and a 415-nm/280 nm ratio of 1.15. After reduction, EPO showed two bands on SDS-PAGE of m.w. 52,000 and 15,000, respectively. On gel filtration, the unreduced protein had a m.w. of approximately 77,000. Amino acid analyses showed a high content of arginine and aspartic acid. Monospecific antibodies to EPO were prepared in rabbits, and a specific radioimmunoassay was developed. There was an almost linear correlation between the content of EPO measured by the radioimmunoassay and the number of eosinophils in a mixed cell extract from reference material, indicating the eosinophil origin of EPO. The content of EPO was estimated to be 15.0 micrograms/10(6) eosinophils. | |
| 653 | a Amino Acids/analysis | |
| 653 | a Antigen-Antibody Reactions | |
| 653 | a Chromatography; Gel | |
| 653 | a Eosinophil Peroxidase | |
| 653 | a Eosinophilia/enzymology | |
| 653 | a Eosinophils/*enzymology | |
| 653 | a Humans | |
| 653 | a Immunodiffusion | |
| 653 | a Leukocyte Count | |
| 653 | a Molecular Weight | |
| 653 | a Peroxidases/analysis/*isolation & purification/metabolism | |
| 653 | a Radioimmunoassay | |
| 653 | a Research Support; Non-U.S. Gov't | |
| 653 | a MEDICINE | |
| 653 | a MEDICIN | |
| 700 | 1 | a Peterson, C Gu Uppsala universitet,Institutionen för medicinska vetenskaper4 aut |
| 700 | 1 | a Venge, Pu Uppsala universitet,Institutionen för medicinska vetenskaper4 aut0 (Swepub:uu)pervenge |
| 710 | 2 | a Uppsala universitetb Institutionen för medicinska vetenskaper4 org |
| 773 | 0 | t Journal of Immunologyg 134:3, s. 1875-1879q 134:3<1875-1879x 0022-1767x 1550-6606 |
| 856 | 4 | u http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=3918110&dopt=Citation |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-74328 |
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