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Cellular uptake of ...
Cellular uptake of radioiodine delivered by trastuzumab can be modified by the addition of epidermal growth factor.
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- Nordberg, Erika (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,Enheten för biomedicinsk strålningsvetenskap
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- Steffen, Ann-Charlott (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,Enheten för biomedicinsk strålningsvetenskap
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- Persson, Mikael (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,Enheten för biomedicinsk strålningsvetenskap
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- Sundberg, Åsa L. (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,Enheten för biomedicinsk strålningsvetenskap
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- Carlsson, Jörgen (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,Enheten för biomedicinsk strålningsvetenskap
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- Glimelius, Bengt (författare)
- Karolinska Institutet,Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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(creator_code:org_t)
- 2005-03-12
- 2005
- Engelska.
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Ingår i: European Journal of Nuclear Medicine and Molecular Imaging. - : Springer Science and Business Media LLC. - 1619-7070 .- 1619-7089. ; 32:7, s. 771-7
- Relaterad länk:
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http://www.ncbi.nlm....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- PURPOSE: The purpose of this study was to analyse whether non-radiolabelled epidermal growth factor (EGF) can modify the cellular uptake of 125I when delivered as [125I]trastuzumab. 125I was used as a marker for the diagnostically and therapeutically more interesting isotopes 123I (SPECT), 124I (PET) and 131I (therapy). METHODS: The cell-associated radioactivity was measured in squamous carcinoma A431 cells following addition of [125I]trastuzumab. Different concentrations of [125I]trastuzumab and unlabelled EGF were used, and the total, membrane-bound and internalised radioactivity was measured. We also analysed how EGF and trastuzumab affected the cell growth. RESULTS: It was generally found that the cellular 125I uptake was decreased by the addition of EGF when [125I]trastuzumab was added for short incubation times. However, if the incubation times were longer, EGF increased the 125I uptake. This shift came earlier when higher [125I]trastuzumab concentrations were applied. The addition of EGF also influenced cell proliferation, and concentrations above 10 ng/ml reduced cell growth by approximately 20% after 24 h of incubation. CONCLUSION: By adding unlabelled EGF, it was possible to modify the cellular uptake of [125I]trastuzumab. This points towards new approaches for the modification of radionuclide uptake in EGFR- and HER2-positive tumours.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)
Nyckelord
- Antibodies; Monoclonal/*administration & dosage/chemistry
- Antineoplastic Agents/*administration & dosage
- Cell Line; Tumor
- Cell Membrane/metabolism
- Cell Proliferation
- Dose-Response Relationship; Drug
- Epidermal Growth Factor/*administration & dosage/metabolism
- Humans
- Iodine Radioisotopes/*pharmacokinetics
- Ligands
- Positron-Emission Tomography
- Receptor; Epidermal Growth Factor/metabolism
- Receptor; erbB-2/metabolism
- Time Factors
- Tomography; Emission-Computed; Single-Photon
- Tumor Markers; Biological
- Radiation biology
- Strålningsbiologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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