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Separation of Pharm...
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Lodén, Henrik,1972-Uppsala universitet,Avdelningen för analytisk farmaceutisk kemi
(författare)
Separation of Pharmaceuticals by Capillary Electrophoresis using Partial Filling and Multiple-injections
Förlag, utgivningsår, omfång ...
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Uppsala :Acta Universitatis Upsaliensis,2008
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60 s.
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-8841
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ISBN:9789155472115
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8841URI
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:vet swepub-contenttype
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Ämneskategori:dok swepub-publicationtype
Serie
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Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy,1651-6192 ;75
Anmärkningar
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Different multiple-injection methodologies and the partial filling technique (PFT) have been utilized for separation of pharmaceuticals by capillary elec-trophoresis. In multiple-injection capillary zone electrophoresis (MICZE), the samples and all standards, used for construction of the calibration curve, are analyzed within a single run. Four different modes of MICZE have been described by means of equations, which were experimentally verified. The developed equations facilitate the transfer from conventional single-injection CZE to one or more of these MICZE-modes, depending on the selectivity between the analyte and the injection marker. The applicability of two of these modes was then demonstrated by quantification of buserelin and salbutamol, re-spectively in commercially available pharmaceutical products. The content of buserelin in an injection solution was determined to 0.94 mg/ml, which only deviated slightly from the declared concentration (1 mg/ml). An alter-native mode of MICZE, offering a higher number of sequential sample injec-tions, was then utilized for single-run determination of salbutamol in 15 tab-lets, with a labelled content of 8 mg. The average content of the tablets was determined to 7.8 mg, with an intra-tablet variation of 3 % or less. Moreover, UV- and mass-spectrometric detection of enantiomeric amines, resolved by non-aqueous capillary electrophoresis (NACE), was demon-strated. Separation of enantiomeric amines was achieved using the chiral selector (-)-2,3:4,6-di-O-isopropylidene-2-keto-L-gulonic acid, (-)-DIKGA. Introduction of the non-volatile (-)-DIKGA into the mass-spectrometer was avoided by using the PFT, where the capillary is only partially filled with electrolyte containing the chiral selector.
Ämnesord och genrebeteckningar
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Capillary Coating
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Chiral Separation
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Enantiomers
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Mass-spectrometric Detection
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Method Validation
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Multiple-injection Capillary Zone Electrophoresis
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Non-aqueous Capillary Electrophoresis
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Organic Solvents
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Pharmaceutical Analysis
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Quantitative Analysis
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Pettersson, Curt
(preses)
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Arvidsson, Torbjörn
(preses)
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Amini, Ahmad
(preses)
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Stefansson, Morgan,DocentAstraZeneca R&D, Mölndal
(opponent)
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Uppsala universitetAvdelningen för analytisk farmaceutisk kemi
(creator_code:org_t)
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