Sökning: onr:"swepub:oai:DiVA.org:uu-89453" > In vitro-in vivo co...
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000 | 02848naa a2200301 4500 | |
001 | oai:DiVA.org:uu-89453 | |
003 | SwePub | |
008 | 011003s2003 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-894532 URI |
024 | 7 | a https://doi.org/10.1002/jps.102032 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Welin-Berger, K.u Uppsala universitet,Institutionen för farmaci4 aut |
245 | 1 0 | a In vitro-in vivo correlation in man of a topically applied local anesthetic agent using numerical convolution and deconvolution |
264 | 1 | b Elsevier BV,c 2003 |
338 | a print2 rdacarrier | |
520 | a The aim of this study was to evaluate the relevance of the in vitro permeation method used at our laboratory in predicting in vivo dermal and transdermal absorption. Two different emulsions, a submicron oil-in-water (o/w) emulsion and a semisolid water-in-oil (w/o) emulsion, containing a model compound were investigated. The in vitro permeation rate of the compound from these emulsions was measured using static diffusion cells with human skin as membrane. The emulsions were allowed to remain in contact with the skin in the donor chamber for 15, 60, and 240 min. The study was monitored for 240 min and the steady state flux was calculated. The systemic concentration of the compound was measured in vivo as a function of time after dermal application to healthy volunteers with 15 and 60 min of application. A short-lasting i.v. infusion study in healthy volunteers was used to simulate the i.v. bolus dose. Numerical convolution was used to predict the in vivo plasma concentration of the compound while the in vivo absorption rate of the compound was estimated using numerical deconvolution. To establish correlation, the predicted in vivo flux was compared with the corresponding observed in vitro parameter after adjusting for the lag time. No major differences were seen in the systemic plasma levels between the two emulsions, which is in close agreement with the steady state flux measured in vitro. A linear correlation representing a point-to-point relationship was established for each of the investigated formulations and application times. The longer application time was predicted more accurately for both emulsions. | |
700 | 1 | a Neelissen, J.A.M.4 aut |
700 | 1 | a Emanuelsson, B.M.4 aut |
700 | 1 | a Björnsson, M.A.4 aut |
700 | 1 | a Gjellan, K.4 aut |
710 | 2 | a Uppsala universitetb Institutionen för farmaci4 org |
773 | 0 | t International Journal of Pharmaceuticsd : Elsevier BVg 92:2, s. 398-406q 92:2<398-406x 0378-5173x 1873-3476x 0022-3549 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-89453 |
856 | 4 8 | u https://doi.org/10.1002/jps.10203 |
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