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Human urinary bladd...
Human urinary bladder carcinomas express adenovirus attachment and internalization receptors
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- Loskog, Angelica (författare)
- Uppsala universitet,Enheten för onkologi,Gen och immunoterapi gruppen
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Hedlund, Titti (författare)
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- Wester, Kenneth (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,Experimentell urologi
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de la Torre, Manuel (författare)
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- Philipson, Lennart (författare)
- Karolinska Institutet
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- Malmström, Per-Uno (författare)
- Uppsala universitet,Institutionen för kirurgiska vetenskaper,Experimentell urologi
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- Tötterman, Thomas H. (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,Gen och immunoterapi gruppen
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(creator_code:org_t)
- 2002-04-25
- 2002
- Engelska.
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Ingår i: Gene Therapy. - : Springer Science and Business Media LLC. - 0969-7128 .- 1476-5462. ; 9:9, s. 547-553
- Relaterad länk:
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- The use of adenoviral vectors as potent gene delivery systems requires expression of the Coxsackievirus/adenovirus receptor (CVADR) on the target cell surface. This receptor is important for virus attachment to the cell surface. For effective internalization of the vector into the target cell the integrins alpha(v)beta(3) and/or alpha(v)beta(5) are needed. Since there have been reports of loss of CVADR in bladder cancer cell lines, we wanted to investigate the expression of this receptor in bladder carcinoma biopsies. Surgical biopsies, as well as five human bladder cancer cell lines, were analyzed for expression of CVADR, the integrins alpha(v)beta(3) and alpha(v)beta(5) and MHC class I. Further, we studied the ability to transduce these cell lines using adenoviral vectors. Immunohistochemistry revealed that all biopsies (27/27) were positive for CVADR. Some variation in expression was evident, and superficially growing tumors stained more strongly than invasive ones. Most human tumors expressed the integrin alpha(v)beta(5) (14/24), whereas integrin alpha(v)beta(3) was less frequently seen (3/20). The established cell lines were efficiently transduced with adenoviral vectors, and transduction could be reduced with anti-CVADR antibodies. The abundance of appropriate viral receptors on tumor biopsy cells is a further argument for using adenoviral vectors in gene therapy of bladder cancer.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- adenovirus
- CVADR
- CAR
- integrins
- bladder cancer
- Immunology
- Immunologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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