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Total Lipid Extract...
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Isaac, GiorgisUppsala universitet,Avdelningen för analytisk kemi
(författare)
Total Lipid Extraction of Homogenized and Intact Lean Fish Muscles Using Pressurized Fluid Extraction and Batch Extraction Technique
- Artikel/kapitelEngelska2005
Förlag, utgivningsår, omfång ...
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2005-06-09
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American Chemical Society (ACS),2005
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-93095
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-93095URI
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https://doi.org/10.1021/jf0501286DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The reliability and efficiency of pressurized fluid extraction (PFE) technique for the extraction of total lipid content from cod and the effect of sample treatment on the extraction efficiency have been evaluated. The results were compared with two liquid-liquid extraction methods, traditional and modified methods according to Jensen. Optimum conditions were found to be with 2-propanol/n-hexane (65:35, v/v) as a first and n-hexane/diethyl ether (90:10, v/v) as a second solvent, 115 oC, and 10 min of static time. PFE extracts were cleaned up using the same procedure as in the methods according to Jensen. When total lipid yields obtained from homogenized cod muscle using PFE were compared yields obtained with original and modified Jensen methods, PFE gave significantly higher yields, ~10% higher (t test, P < 0.05). Infrared and NMR spectroscopy suggested that the additional material that inflates the gravimetric results is rather homogeneus and is primarily consists of phospholipid with headgroups of inositidic and/or glycosidic nature. The comparative study demonstrated that PFE is an alternative suitable technique to extract total lipid content from homogenized cod (lean fish) and herring (fat fish) muscle showing a precision comparable to that obtained with the traditional and modified Jensen methods. Despite the necessary cleanup step, PFE showed important advantages in the solvent consumption was cut by ~50% and automated extraction was possible.
Ämnesord och genrebeteckningar
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Lean fish
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phospholipids
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pressurized fluid extraction
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PFE
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total lipid content
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Waldebäck, MonicaUppsala universitet,Avdelningen för analytisk kemi
(författare)
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Eriksson, Ulla
(författare)
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Odham, Göran
(författare)
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Markides, Karin E.Uppsala universitet,Avdelningen för analytisk kemi
(författare)
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Uppsala universitetAvdelningen för analytisk kemi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Agricultural and Food Chemistry: American Chemical Society (ACS)53:14, s. 5506-55120021-85611520-5118
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