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Proteomic evaluatio...
Proteomic evaluation of neonatal exposure to 2,2,4,4,5-pentabromodiphenyl ether
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- Alm, Henrik (författare)
- Uppsala universitet,Avdelningen för toxikologi
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- Scholz, Birger (författare)
- Uppsala universitet,Avdelningen för toxikologi
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- Fischer, Celia (författare)
- Uppsala universitet,Ekotoxikologi
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- Kultima, Kim (författare)
- Uppsala universitet,Avdelningen för toxikologi
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- Viberg, Henrik (författare)
- Uppsala universitet,Ekotoxikologi
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- Eriksson, Per (författare)
- Uppsala universitet,Ekotoxikologi
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- Dencker, Lennart (författare)
- Uppsala universitet,Avdelningen för toxikologi
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- Stigson, Michael (författare)
- Uppsala universitet,Avdelningen för toxikologi
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(creator_code:org_t)
- Environmental Health Perspectives, 2006
- 2006
- Engelska.
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Ingår i: Journal of Environmental Health Perspectives. - : Environmental Health Perspectives. - 0091-6765 .- 1552-9924. ; 114:2, s. 254-259
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Exposure to the brominated flame retardant 2,2 ,4,4 ,5-pentabromodiphenyl ether (PBDE-99) during the brain growth spurt disrupts normal brain development in mice and results in disturbed spontaneous behavior in adulthood. The neurodevelopmental toxicity of PBDE-99 has been reported to affect the cholinergic and catecholaminergic systems. In this study we use a proteomics approach to study the early effect of PBDE-99 in two distinct regions of the neonatal mouse brain, the striatum and the hippocampus. A single oral dose of PBDE-99 (12 mg/kg body weight) or vehicle was administered to male NMRI mice on neonatal day 10, and the striatum and the hippocampus were isolated. Using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE), we found 40 and 56 protein spots with significantly (p < 0.01) altered levels in the striatum and the hippocampus, respectively. We used matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF-MS) to determine the protein identity of 11 spots from the striatum and 10 from the hippocampus. We found that the levels of proteins involved in neurodegeneration and neuroplasticity (e.g., Gap-43/neuromodulin, stathmin) were typically altered in the striatum, and proteins involved in metabolism and energy production [e.g., alpha-enolase; gamma-enolase; ATP synthase, H+ transporting, mitochondrial F1 complex, beta subunit (Atp5b); and alpha-synuclein] were typically altered in the hippocampus. Interestingly, many of the identified proteins have been linked to protein kinase C signaling. In conclusion, we identify responses to early exposure to PBDE-99 that could contribute to persistent neurotoxic effects. This study also shows the usefulness of proteomics to identify potential biomarkers of developmental neurotoxicity of organohalogen compounds.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Nyckelord
- 2D-GE
- brain development
- brain growth spurt
- MALDI–ToF–MS
- neonatal
- neurodegeneration
- PBDE-99
- PKC
- proteomics
- PHARMACY
- FARMACI
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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