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Food-induced expres...
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Bengtsson, Magnus W.Uppsala universitet,Fysiologi
(författare)
Food-induced expression of orexin receptors in rat duodenal mucosa regulates the bicarbonate secretory response to orexin-A
- Artikel/kapitelEngelska2007
Förlag, utgivningsår, omfång ...
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American Physiological Society,2007
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-97103
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-97103URI
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https://doi.org/10.1152/ajpgi.00514.2006DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Presence of appetite-regulating peptides orexin-A and orexin-B in mucosal endocrine cells suggests a role in physiological control of the intestine. Our aim was to characterize orexin-induced stimulation of duodenal bicarbonate secretion and modulation of secretory responses and mucosal orexin receptors by overnight food deprivation. Lewis x Dark Agouti rats were anesthetized and proximal duodenum cannulated in situ. Mucosal bicarbonate secretion (pH stat) and mean arterial blood pressure were continuously recorded. Orexin-A was administered intra-arterially close to the duodenum, intraluminally, or into the brain ventricles. Total RNA was extracted from mucosal specimens, reverse transcribed to cDNA and expression of orexin receptors 1 and 2 (OX1 and OX2) measured by quantitative real-time PCR. OX1 protein was measured by Western blot. Intra-arterial orexin-A (60–600 nmol·h–1·kg–1) increased (P < 0.01) the duodenal secretion in fed but not in fasted animals. The OX1 receptor antagonist SB-334867, which was also found to have a partial agonist action, abolished the orexin-induced secretory response but did not affect secretion induced by the muscarinic agonist bethanechol. Atropine, in contrast, inhibited bethanechol but not orexin-induced secretion. Orexin-A infused into the brain ventricles (2–20 nmol·kg–1·h–1) or added to luminal perfusate (1.0–100 nM) did not affect secretion, indicating that orexin-A acts peripherally and at basolateral receptors. Overnight fasting decreased mucosal OX1 and OX2 mRNA expression (P < 0.01) as well as OX1 protein expression (P < 0.05). We conclude that stimulation of secretion by orexin-A may involve both receptor types and is independent of cholinergic pathways. Intestinal OX receptors and secretory responses are markedly related to food intake.
Ämnesord och genrebeteckningar
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bicarbonate secretion
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enteroendocrine cells
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fed and fasting state
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perfused duodenum in situ
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TRH
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MEDICINE
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MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Mäkelä, Kari
(författare)
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Sjöblom, MarkusUppsala universitet,Fysiologi(Swepub:uu)msj20812
(författare)
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Uotila, Sanna
(författare)
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Åkerman, Karl E. O.Uppsala universitet,Fysiologi
(författare)
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Herzig, Karl-Heinz
(författare)
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Flemström, GunnarUppsala universitet,Fysiologi(Swepub:uu)gfl07049
(författare)
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Uppsala universitetFysiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:American Journal of Physiology - Gastrointestinal and Liver Physiology: American Physiological Society293:2, s. G501-G5090193-18571522-1547
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