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Sökning: onr:"swepub:oai:DiVA.org:uu-97103" > Food-induced expres...

  • Bengtsson, Magnus W.Uppsala universitet,Fysiologi (författare)

Food-induced expression of orexin receptors in rat duodenal mucosa regulates the bicarbonate secretory response to orexin-A

  • Artikel/kapitelEngelska2007

Förlag, utgivningsår, omfång ...

  • American Physiological Society,2007
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:uu-97103
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-97103URI
  • https://doi.org/10.1152/ajpgi.00514.2006DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Presence of appetite-regulating peptides orexin-A and orexin-B in mucosal endocrine cells suggests a role in physiological control of the intestine. Our aim was to characterize orexin-induced stimulation of duodenal bicarbonate secretion and modulation of secretory responses and mucosal orexin receptors by overnight food deprivation. Lewis x Dark Agouti rats were anesthetized and proximal duodenum cannulated in situ. Mucosal bicarbonate secretion (pH stat) and mean arterial blood pressure were continuously recorded. Orexin-A was administered intra-arterially close to the duodenum, intraluminally, or into the brain ventricles. Total RNA was extracted from mucosal specimens, reverse transcribed to cDNA and expression of orexin receptors 1 and 2 (OX1 and OX2) measured by quantitative real-time PCR. OX1 protein was measured by Western blot. Intra-arterial orexin-A (60–600 nmol·h–1·kg–1) increased (P < 0.01) the duodenal secretion in fed but not in fasted animals. The OX1 receptor antagonist SB-334867, which was also found to have a partial agonist action, abolished the orexin-induced secretory response but did not affect secretion induced by the muscarinic agonist bethanechol. Atropine, in contrast, inhibited bethanechol but not orexin-induced secretion. Orexin-A infused into the brain ventricles (2–20 nmol·kg–1·h–1) or added to luminal perfusate (1.0–100 nM) did not affect secretion, indicating that orexin-A acts peripherally and at basolateral receptors. Overnight fasting decreased mucosal OX1 and OX2 mRNA expression (P < 0.01) as well as OX1 protein expression (P < 0.05). We conclude that stimulation of secretion by orexin-A may involve both receptor types and is independent of cholinergic pathways. Intestinal OX receptors and secretory responses are markedly related to food intake.

Ämnesord och genrebeteckningar

  • bicarbonate secretion
  • enteroendocrine cells
  • fed and fasting state
  • perfused duodenum in situ
  • TRH
  • MEDICINE
  • MEDICIN

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Mäkelä, Kari (författare)
  • Sjöblom, MarkusUppsala universitet,Fysiologi(Swepub:uu)msj20812 (författare)
  • Uotila, Sanna (författare)
  • Åkerman, Karl E. O.Uppsala universitet,Fysiologi (författare)
  • Herzig, Karl-Heinz (författare)
  • Flemström, GunnarUppsala universitet,Fysiologi(Swepub:uu)gfl07049 (författare)
  • Uppsala universitetFysiologi (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:American Journal of Physiology - Gastrointestinal and Liver Physiology: American Physiological Society293:2, s. G501-G5090193-18571522-1547

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