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Novel genes in cell...
Novel genes in cell cycle control and lipid metabolism with dynamically regulated binding sites for sterol regulatory element-binding protein 1 and RNA polymerase II in HepG2 cells detected by chromatin immunoprecipitation with microarray detection
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- Motallebipour, Mehdi (författare)
- Uppsala universitet,Institutionen för genetik och patologi,Wadelius
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- Enroth, Stefan (författare)
- Uppsala universitet,Centrum för bioinformatik,Komorowski
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- Punga, Tanel (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning,Institutionen för medicinsk biokemi och mikrobiologi,Ericsson
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- Ameur, Adam (författare)
- Uppsala universitet,Centrum för bioinformatik,Komorowski
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- Koch, Christoph (författare)
- Wellcome Trust Sanger Institute
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- Dunham, Ian (författare)
- Wellcome Trust Sanger Institute
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- Komorowski, Jan (författare)
- Uppsala universitet,Centrum för bioinformatik,Komorowski
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- Ericsson, Johan (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning,Ericsson
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- Wadelius, Claes (författare)
- Uppsala universitet,Institutionen för genetik och patologi,Wadelius
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(creator_code:org_t)
- 2009-02-17
- 2009
- Engelska.
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Ingår i: The FEBS Journal. - : Wiley. - 1742-464X .- 1742-4658. ; 276:7, s. 1878-1890
- Relaterad länk:
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https://febs.onlinel...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Sterol regulatory element-binding proteins 1 and 2 (SREBP-1 and SREBP-2) are important regulators of genes involved in cholesterol and fatty acid metabolism, but have also been implicated in the regulation of the cell cycle and have been associated with the pathogenesis of type 2 diabetes, atherosclerosis and obesity, among others. In this study, we aimed to characterize the binding sites of SREBP-1 and RNA polymerase II through chromatin immunoprecipitation and microarray analysis in 1% of the human genome, as defined by the Encyclopaedia of DNA Elements consortium, in a hepatocellular carcinoma cell line (HepG2). Our data identified novel binding sites for SREBP-1 in genes directly or indirectly involved in cholesterol metabolism, e.g. apolipoprotein C-III (APOC3). The most interesting biological findings were the binding sites for SREBP-1 in genes for host cell factor C1 (HCFC1), involved in cell cycle regulation, and for filamin A (FLNA). For RNA polymerase II, we found binding sites at classical promoters, but also in intergenic and intragenic regions. Furthermore, we found evidence of sterol-regulated binding of SREBP-1 and RNA polymerase II to HCFC1 and FLNA. From the results of this work, we infer that SREBP-1 may be involved in processes other than lipid metabolism.
Nyckelord
- HCFC1
- human
- intragenic binding
- sterolregulated
- transcriptional regulation
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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