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Quantitative chemic...
Quantitative chemical analysis of single cells.
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Heien, Michael L (författare)
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- Ewing, Andrew G, 1957 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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(creator_code:org_t)
- ISBN 9781597454834
- 2009-05-07
- 2009
- Engelska.
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Ingår i: Methods in molecular biology book series, Volume 544, Part 2: Micro and Nano Technologies in Bioanalysis (eds Robert S. Foote, James Weifu Lee). - Totowa, NJ : Humana Press. - 9781597454834 ; , s. 153-62
- Relaterad länk:
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http://dx.doi.org/10...
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Abstract
Ämnesord
Stäng
- Exocytosis, the fusion of intracellular vesicles with the membrane and subsequent release of vesicular contents, is important in intercellular communication. The release event is a rapid process (milliseconds), hence detection of released chemicals requires a detection scheme that is both sensitive and has rapid temporal dynamics. Electrochemistry at carbon-fiber microelectrodes allows time-resolved exocytosis of electroactive catecholamines to be observed at very low levels. When coupled with constant-potential amperometry, the number of molecules released and the kinetics of quantal release can be determined. The rapid response time (milliseconds) of microelectrodes makes them well suited for monitoring the dynamic process of exocytosis.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Animals
- Cells
- chemistry
- secretion
- Electrochemical Techniques
- instrumentation
- methods
- Exocytosis
- Microelectrodes
- PC12 Cells
- Rats
- Cells
Publikations- och innehållstyp
- vet (ämneskategori)
- kap (ämneskategori)
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