Sökning: onr:"swepub:oai:gup.ub.gu.se/106076" > Effects of prostagl...
Fältnamn | Indikatorer | Metadata |
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000 | 04382naa a2200685 4500 | |
001 | oai:gup.ub.gu.se/106076 | |
003 | SwePub | |
008 | 240910s2007 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:umu-118192 | |
024 | 7 | a https://gup.ub.gu.se/publication/1060762 URI |
024 | 7 | a https://doi.org/10.1016/j.plefa.2007.09.0022 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1181922 URI |
040 | a (SwePub)gud (SwePub)umu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Kaneko, H4 aut |
245 | 1 0 | a Effects of prostaglandin E2 and lipopolysaccharide on osteoclastogenesis in RAW 264.7 cells. |
264 | 1 | a Edinburgh :b Elsevier BV,c 2007 |
520 | a INTRODUCTION: Prostaglandins (PGs) can act on both hematopoietic and osteoblastic lineages to enhance osteoclast formation. METHODS: We examined PGE2 stimulated osteoclastogenesis in RAW 264.7 cells and the role of endogenous PGE2 in lipopolysaccharide (LPS) stimulated osteoclastogenesis. RESULTS: RANKL (1-100 ng/ml) increased formation of osteoclasts, defined as tartrate resistant acid phosphatase multinucleated cells, with peak effects at 30 ng/ml. Addition of PGE2 (0.01-1.0 microM) to RANKL (30 ng/ml) dose dependently increased osteoclast number 30-150%. Use of NS-398 (0.1 microM) or indomethacin (Indo, 1.0 micro M) to block endogenous PG synthesis had little effect on the response to RANKL alone but significantly decreased the response to PGE2. Addition of LPS (100 ng/ml) to RANKL increased osteoclast number 50%, and this response was significantly decreased by NS-398 and Indo. RANKL and PGE2 produced small, additive increases in COX-2 mRNA levels, while LPS produced a larger increase. PG release into the medium was not increased by RANKL and PGE2 but markedly increased by LPS. CONCLUSION: We conclude that RANKL stimulated osteoclastogenesis can be enhanced by PGE2 and LPS though direct effects on the hematopoietic cell lineage and that these effects may be mediated in part by induction of COX-2 and enhanced intracellular PG production. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Odontologi0 (SwePub)302162 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Dentistry0 (SwePub)302162 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Fysiologi0 (SwePub)301062 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Physiology0 (SwePub)301062 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Cellbiologi0 (SwePub)106042 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Cell Biology0 (SwePub)106042 hsv//eng |
653 | a Animals | |
653 | a Cell Line | |
653 | a Tumor | |
653 | a Dinoprostone | |
653 | a pharmacology | |
653 | a Lipopolysaccharides | |
653 | a pharmacology | |
653 | a Mice | |
653 | a Nitrobenzenes | |
653 | a pharmacology | |
653 | a Osteoclasts | |
653 | a drug effects | |
653 | a Prostaglandins F | |
653 | a Synthetic | |
653 | a pharmacology | |
653 | a RANK Ligand | |
653 | a pharmacology | |
653 | a Sulfonamides | |
653 | a pharmacology | |
653 | a RAW 264.7 macrophages | |
700 | 1 | a Mehrotra, M4 aut |
700 | 1 | a Alander, C4 aut |
700 | 1 | a Lerner, Ulf Hu Umeå universitet,Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin,Institute of Medicine, Department of Internal Medicine,Oral cellbiologi4 aut0 (Swepub:umu)ulle0001 |
700 | 1 | a Pilbeam, C4 aut |
700 | 1 | a Raisz, L4 aut |
710 | 2 | a Göteborgs universitetb Institutionen för medicin, avdelningen för invärtesmedicin4 org |
773 | 0 | t Prostaglandins, leukotrienes, and essential fatty acidsd Edinburgh : Elsevier BVg 77:3-4, s. 181-6q 77:3-4<181-6x 0952-3278x 1532-2823 |
856 | 4 | u https://europepmc.org/articles/pmc2836877?pdf=render |
856 | 4 8 | u https://gup.ub.gu.se/publication/106076 |
856 | 4 8 | u https://doi.org/10.1016/j.plefa.2007.09.002 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-118192 |
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