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Molecular evolution...
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Persson, JonasLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
(författare)
Molecular evolution of specific human antibody against MUC1 mucin results in improved recognition of the antigen on tumor cells.
- Artikel/kapitelEngelska2009
Förlag, utgivningsår, omfång ...
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Springer Science and Business Media LLC,2009
Nummerbeteckningar
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LIBRIS-ID:oai:gup.ub.gu.se/106466
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https://gup.ub.gu.se/publication/106466URI
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https://doi.org/10.1159/000240634DOI
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https://lup.lub.lu.se/record/1438914URI
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Ämneskategori:art swepub-publicationtype
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The MUC1 mucin is differentially expressed and glycosylated in cancer tissue as opposed to healthy tissue. Due to these differences, MUC1 is considered a potential biomarker suitable for cancer diagnosis and therapy. In a previous study, the human MUC1-specific antibody 12ESC-6 was able to bind a sequence variant of the tandem repeat of MUC1 that is not recognized by many other MUC1-specific antibodies. It was also found to bind efficiently to MUC1-carrying cells. We have now used 12ESC-6 as starting point for random mutagenesis to isolate variants with improved ability to bind MUC1 in human tumor tissue. The resulting 12ESC-6 variants were shown to recognize not only the naked MUC1 tandem repeat but even more so glycosylated variants thereof, in particular those carrying the GalNAc (Tn) glycoform. Selected variants of 12ESC-6 demonstrated improved staining of MUC1 on cell lines using flow cytometry and improved staining of the antigen in breast tumor tissue by immunohistochemistry. Molecular evolution and specific fine-tuning thus have the potential to improve the performance of antibody specificities targeting tumor-associated epitopes on MUC1 mucin.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Bäckström, Malin,1967Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xbamal
(författare)
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Johansson, H
(författare)
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Jirström, KarinLund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)pat-kji
(författare)
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Hansson, Gunnar C.,1951Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xhagun
(författare)
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Ohlin, MatsLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)immt-moh
(författare)
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Institutionen för immunteknologiInstitutioner vid LTH
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine: Springer Science and Business Media LLC30:4, s. 221-311423-03801010-4283
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Ingår i:Tumor Biology: Springer Science and Business Media LLC30:4, s. 221-311010-4283
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