Sökning: onr:"swepub:oai:gup.ub.gu.se/106725" >
Analysis of monosaccharide composition of mucin oligosaccharide alditols by high-performance anion-exchange chromatography.
-
- Karlsson, Niclas G., 1966 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
-
- Hansson, Gunnar C., 1951 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
-
(creator_code:org_t)
- Elsevier BV, 1995
- Engelska.
-
Ingår i: Analytical biochemistry. - : Elsevier BV. - 0003-2697. ; 224:2, s. 538-41
- Relaterad länk:
-
https://gup.ub.gu.se...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- A simple one-step method for the analysis of monosaccharides including galactosaminitol after acidic hydrolysis is described. The hydrolyzate was re-N-acetylated and analyzed by high-performance anion-exchange chromatography and the sugars were detected by a pulsed amperometric detector. The method was applied on a mixture of neutral oligosaccharides released from mucin glycopeptides of rat small intestine by alkaline borohydride. Sugars were detected down to the nanomole range and the results were compared with monosaccharide compositional analysis performed by gas chromatography of acetylated alditols.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Acetylation
- Animals
- Anions
- Boranes
- Chromatography
- High Pressure Liquid
- Chromatography
- Ion Exchange
- Hydrolysis
- Intestine
- Small
- chemistry
- Monosaccharides
- analysis
- Mucins
- chemistry
- Oligosaccharides
- analysis
- Rats
- Sugar Alcohols
- analysis
- Trifluoroacetic Acid
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)