Estimation of the PCR efficiency based on a size-dependent modelling of the amplification process

• We propose a stochastic modelling of the PCR amplification process by a size-dependent branching process starting as a supercritical Bienaymé-Galton-Watson transient phase and then having a saturation near-critical size-dependent phase. This model based on the concept of saturation allows one to estimate the probability of replication of a DNA molecule at each cycle of a single PCR trajectory with a very good accuracy.

Ämnesord

NATURVETENSKAP  -- Matematik -- Sannolikhetsteori och statistik (hsv//swe)

NATURAL SCIENCES  -- Mathematics -- Probability Theory and Statistics (hsv//eng)

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