Sökning: onr:"swepub:oai:gup.ub.gu.se/111599" > Impaired insulin ex...
Fältnamn | Indikatorer | Metadata |
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000 | 05695naa a2200853 4500 | |
001 | oai:gup.ub.gu.se/111599 | |
003 | SwePub | |
008 | 240910s2009 | |||||||||||000 ||eng| | |
009 | oai:lup.lub.lu.se:01d3fb3a-e8f9-468a-ac46-d177be2f26e6 | |
024 | 7 | a https://gup.ub.gu.se/publication/1115992 URI |
024 | 7 | a https://doi.org/10.1210/en.2008-04752 DOI |
024 | 7 | a https://lup.lub.lu.se/record/14627492 URI |
040 | a (SwePub)gud (SwePub)lu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Olofsson, Charlotta S,d 1971u Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology4 aut0 (Swepub:gu)xoloch |
245 | 1 0 | a Impaired insulin exocytosis in neural cell adhesion molecule-/- mice due to defective reorganization of the submembrane F-actin network. |
264 | c 2009-02-12 | |
264 | 1 | b The Endocrine Society,c 2009 |
520 | a The neural cell adhesion molecule (NCAM) is required for cell type segregation during pancreatic islet organogenesis. We have investigated the functional consequences of ablating NCAM on pancreatic beta-cell function. In vivo, NCAM(-/-) mice exhibit impaired glucose tolerance and basal hyperinsulinemia. Insulin secretion from isolated NCAM(-/-) islets is enhanced at glucose concentrations below 15 mM but inhibited at higher concentrations. Glucagon secretion from pancreatic alpha-cells evoked by low glucose was also severely impaired in NCAM(-/-) islets. The diminution of insulin secretion is not attributable to defective glucose metabolism or glucose sensing (documented as glucose-induced changes in intracellular Ca(2+) and K(ATP)-channel activity). Resting K(ATP) conductance was lower in NCAM(-/-) beta-cells than wild-type cells, and this difference was abolished when F-actin was disrupted by cytochalasin D (1 muM). In wild-type beta-cells, the submembrane actin network disassembles within 10 min during glucose stimulation (30 mM), an effect not seen in NCAM(-/-) beta-cells. Cytochalasin D eliminated this difference and normalized insulin and glucagon secretion in NCAM(-/-) islets. Capacitance measurements of exocytosis indicate that replenishment of the readily releasable granule pool is suppressed in NCAM(-/-) alpha- and beta-cells. Our data suggest that remodeling of the submembrane actin network is critical to normal glucose regulation of both insulin and glucagon secretion. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Fysiologi0 (SwePub)301062 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Physiology0 (SwePub)301062 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Endokrinologi och diabetes0 (SwePub)302052 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Endocrinology and Diabetes0 (SwePub)302052 hsv//eng |
653 | a Actins | |
653 | a metabolism | |
653 | a Adenosine Triphosphate | |
653 | a metabolism | |
653 | a Animals | |
653 | a Exocytosis | |
653 | a physiology | |
653 | a Female | |
653 | a Glucagon | |
653 | a secretion | |
653 | a Glucose | |
653 | a physiology | |
653 | a Glucose Intolerance | |
653 | a genetics | |
653 | a Insulin | |
653 | a physiology | |
653 | a secretion | |
653 | a Islets of Langerhans | |
653 | a physiology | |
653 | a Mice | |
653 | a Mice | |
653 | a Knockout | |
653 | a Neural Cell Adhesion Molecules | |
653 | a deficiency | |
653 | a Patch-Clamp Techniques | |
653 | a Potassium Channels | |
653 | a Inwardly Rectifying | |
653 | a metabolism | |
700 | 1 | a Håkansson, Joakim4 aut |
700 | 1 | a Salehi, Albert4 aut |
700 | 1 | a Bengtsson, Martin4 aut |
700 | 1 | a Galvanovskis, Juris4 aut |
700 | 1 | a Partridge, Chris4 aut |
700 | 1 | a SörhedeWinzell, Maria4 aut |
700 | 1 | a Xian, Xiaojieu Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)med-xjx |
700 | 1 | a Eliasson, Lena4 aut |
700 | 1 | a Lundquist, Ingmar4 aut |
700 | 1 | a Semb, Henriku Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)endo-hse |
700 | 1 | a Rorsman, Patrik,d 19594 aut |
700 | 1 | a Sörhede Winzell, Mariau Lund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)medk-msw |
710 | 2 | a Göteborgs universitetb Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi4 org |
773 | 0 | t Endocrinologyd : The Endocrine Societyg 150:7, s. 3067-75q 150:7<3067-75x 1945-7170x 0013-7227 |
856 | 4 | u https://academic.oup.com/endo/article-pdf/150/7/3067/9004311/endo3067.pdf |
856 | 4 | u http://www.ncbi.nlm.nih.gov/pubmed/19213846?dopt=Abstractx freey FULLTEXT |
856 | 4 | u http://dx.doi.org/10.1210/en.2008-0475y FULLTEXT |
856 | 4 8 | u https://gup.ub.gu.se/publication/111599 |
856 | 4 8 | u https://doi.org/10.1210/en.2008-0475 |
856 | 4 8 | u https://lup.lub.lu.se/record/1462749 |
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