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Micropipet-assisted...
Micropipet-assisted formation of microscopic networks of unilamellar lipid bilayer nanotubes and containers
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- Karlsson, Mattias, 1980 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Sott, Kristin, 1974 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Cans, Ann-Sofie, 1971 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Karlsson, Anders (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Karlsson, Roger, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Orwar, Owe, 1964 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2001-10-06
- 2001
- Engelska.
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Ingår i: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 17:22, s. 6754-6758
- Relaterad länk:
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http://dx.doi.org/10...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- We describe a novel micropipet-assisted technique for the construction of complex, surface-immobilized two-dimensional microscopic networks of unilamellar phospholipid bilayer vesicles (1-50 pm in diameter, 10(-15)-10(-12) L) interconnected by lipid nanotubes (100-300 nm in diameter). As starting material for the construction of networks, we used twinned vesicle pairs, one of which is multilamellar and functions as a membrane donor and the other unilamellar and functions as a membrane acceptor upon manipulation. By electromechanical insertion of a pipet tip into the unilamellar vesicle followed by lateral pulling of the micropipet away from the vesicle, a nanotube was formed. Buffer solution contained in the pipet was then injected into the nanotube orifice, forming a vesicle of controlled size that was immobilized on the surface. The networks have controlled connectivity and are well-defined with regard to the container size, angle between nanotube extensions, and nanotube length. The internal fluid composition of individual vesicles is defined during the formation of a network by selection of the solution contained in the micropipet.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- PHOSPHOLIPID-VESICLES
- LIVING CELLS
- PROTEIN
- MOLECULES
- LIPOSOMES
- TUBULES
- FUSION
- LIPOSOMES
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
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Langmuir
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Till lärosätets databas
- Av författaren/redakt...
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Karlsson, Mattia ...
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Sott, Kristin, 1 ...
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Cans, Ann-Sofie, ...
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Karlsson, Anders
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Karlsson, Roger, ...
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Orwar, Owe, 1964
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
- Artiklar i publikationen
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Langmuir
- Av lärosätet
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Göteborgs universitet
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Chalmers tekniska högskola