Mucosal immunization of piglets with purified F18 fimbriae does not protect against F18+ Escherichia coli infection.

• Post-weaning diarrhoea and oedema disease in weaned piglets are caused by infection with F4+ or F18+ Escherichia coli strains. There is no commercial vaccine available, but it is shown that oral immunization of weaned piglets with purified F4 fimbriae induces a protective mucosal immune response. In the present study, piglets were orally and nasally immunized with purified F18 fimbriae in the presence of the mucosal adjuvant LT(R192G) or CTA1-DD, respectively. This immunization could not lead to protection against F18+ E. coli infection. The induced F18-specific immune response was directed towards the major subunit FedA and weakly towards the adhesive subunit FedF. The results of these experiments demonstrate that it is difficult to induce protective immunity against F18+ E. coli using the whole fimbriae due to the low response against the adhesin.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

Nyckelord

Administration

Intranasal

Administration

Oral

Animals

Antibodies

Bacterial

blood

Bacterial Vaccines

administration & dosage

immunology

Drug Administration Routes

Escherichia coli

genetics

isolation & purification

Escherichia coli Infections

prevention & control

veterinary

Escherichia coli Proteins

genetics

immunology

isolation & purification

Feces

microbiology

Fimbriae Proteins

genetics

immunology

isolation & purification

Immunity

Mucosal

immunology

Immunoglobulin G

blood

Immunoglobulin M

blood

Swine

Swine Diseases

immunology

prevention & control

Time Factors

Treatment Failure

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)