Sökning: onr:"swepub:oai:gup.ub.gu.se/125739" > 454 Pyrosequencing ...
Fältnamn | Indikatorer | Metadata |
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000 | 04165naa a2200517 4500 | |
001 | oai:gup.ub.gu.se/125739 | |
003 | SwePub | |
008 | 240528s2010 | |||||||||||000 ||eng| | |
024 | 7 | a https://gup.ub.gu.se/publication/1257392 URI |
024 | 7 | a https://doi.org/10.1111/j.1469-8137.2010.03373.x2 DOI |
040 | a (SwePub)gu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Tedersoo, L.4 aut |
245 | 1 0 | a 454 Pyrosequencing and Sanger sequencing of tropical mycorrhizal fungi provide similar results but reveal substantial methodological biases |
264 | c 2010-07-15 | |
264 | 1 | b Wiley,c 2010 |
520 | a Compared with Sanger sequencing-based methods, pyrosequencing provides orders of magnitude more data on the diversity of organisms in their natural habitat, but its technological biases and relative accuracy remain poorly understood. This study compares the performance of pyrosequencing and traditional sequencing for species’ recovery of ectomycorrhizal fungi on root tips in a Cameroonian rain forest and addresses biases related to multi-template PCR and pyrosequencing analyses. Pyrosequencing and the traditional method yielded qualitatively similar results, but there were slight, but significant, differences that affected the taxonomic view of the fungal community. We found that most pyrosequencing singletons were artifactual and contained a strongly elevated proportion of insertions compared with natural intra- and interspecific variation. The alternative primers, DNA extraction methods and PCR replicates strongly influenced the richness and community composition as recovered by pyrosequencing. Pyrosequencing offers a powerful alternative for the identification of ectomycorrhizal fungi in pooled root samples, but requires careful selection of molecular tools. A well-populated backbone database facilitates the detection of biological and technical artifacts. The pyrosequencing pipeline is available at http://unite.ut.ee/454pipeline.tgz. | |
650 | 7 | a NATURVETENSKAPx Biologix Ekologi0 (SwePub)106112 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Ecology0 (SwePub)106112 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Bioinformatik och systembiologi0 (SwePub)106102 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Bioinformatics and Systems Biology0 (SwePub)106102 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Zoologi0 (SwePub)106082 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Zoology0 (SwePub)106082 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Biologisk systematik0 (SwePub)106122 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biological Systematics0 (SwePub)106122 hsv//eng |
650 | 7 | a NATURVETENSKAPx Geovetenskap och miljövetenskapx Annan geovetenskap och miljövetenskap0 (SwePub)105992 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Earth and Related Environmental Sciencesx Other Earth and Related Environmental Sciences0 (SwePub)105992 hsv//eng |
653 | a 454 massively parallel pyrosequencing; artifacts; biodiversity; community structure; ectomycorrizal fungi; Korup National Park; PCR bias | |
700 | 1 | a Nilsson, R. Henrik,d 1976u Gothenburg University,Göteborgs universitet,Institutionen för växt- och miljövetenskaper,Department of Plant and Environmental Sciences4 aut0 (Swepub:gu)xnihen |
700 | 1 | a Abarenkov, Kessy4 aut |
700 | 1 | a Jairus, Teele4 aut |
700 | 1 | a Sadam, Ave4 aut |
700 | 1 | a Saar, Irja4 aut |
700 | 1 | a Bahram, Mohammad4 aut |
700 | 1 | a Bechem, Eneke4 aut |
700 | 1 | a Chuyong, George4 aut |
700 | 1 | a Kõljalg, Urmas4 aut |
710 | 2 | a Göteborgs universitetb Institutionen för växt- och miljövetenskaper4 org |
773 | 0 | t New Phytologistd : Wileyg 188:1, s. 291-301q 188:1<291-301x 0028-646X |
856 | 4 8 | u https://gup.ub.gu.se/publication/125739 |
856 | 4 8 | u https://doi.org/10.1111/j.1469-8137.2010.03373.x |
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