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Construction and ex...
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Tobias, Joshua,1969Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine
(författare)
Construction and expression of immunogenic hybrid enterotoxigenic Escherichia coli CFA/I and CS2 colonization fimbriae for use in vaccines.
- Artikel/kapitelEngelska2010
Förlag, utgivningsår, omfång ...
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2010-04-20
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Springer Science and Business Media LLC,2010
Nummerbeteckningar
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LIBRIS-ID:oai:gup.ub.gu.se/127734
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https://gup.ub.gu.se/publication/127734URI
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https://doi.org/10.1007/s00253-010-2577-4DOI
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Enterotoxigenic Escherichia coli (ETEC) are an important cause of diarrheal morbidity in developing countries, especially in children and also of traveler's diarrhea. Colonization factors (CFs) of ETEC, like CFA/I and CS2 which are genetically and structurally related, play a substantial role in pathogenicity, and since intestinal-mucosal immune responses against CFs appear to be protective, much effort has focused on the development of a CF-based ETEC vaccine. We have constructed hybrid operons in which the major CS2 subunit-encoding cotA gene was inserted into the CFA/I operon, either replacing (hybrid I) or being added to the major CFA/I subunit-encoding cfaB gene (hybrid II). Using specific monoclonal antibodies against the major subunits of CFA/I and CS2, high levels of surface expression of both fimbrial subunits were shown in E. coli carrying the hybrid II operon. Oral immunization of mice with formalin-killed bacteria expressing hybrid II fimbriae induced strong CFA/I- and CS2-specific serum IgG + IgM and fecal IgA antibody responses, which were higher than those achieved by similar immunization with the reference strains. Bacteria expressing hybrid fimbriae are potential candidate strains in an oral-killed CF-ETEC vaccine, and the approach represents an attractive and novel means of producing a broad-spectrum ETEC vaccine.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Svennerholm, Ann-Mari,1947Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine(Swepub:gu)xsannx
(författare)
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Holmgren, Jan,1944Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine(Swepub:gu)xholja
(författare)
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Lebens, Michael,1956Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine(Swepub:gu)xlebmi
(författare)
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Göteborgs universitetInstitutionen för biomedicin
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Applied microbiology and biotechnology: Springer Science and Business Media LLC87:4, s. 1355-651432-06140175-7598
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