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A mathematical anal...
A mathematical analysis of nuclear intensity dynamics for Mig1-GFP under consideration of bleaching effects and background noise in Saccharomyces cerevisiae
- Artikel/kapitelEngelska2011
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Royal Society of Chemistry (RSC),2011
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LIBRIS-ID:oai:gup.ub.gu.se/161796
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https://gup.ub.gu.se/publication/161796URI
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https://research.chalmers.se/publication/161796URI
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https://doi.org/10.1039/c005305hDOI
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Abstract: Fluorescence microscopy is an imaging technique that provides insights into signal transduction pathways through the generation of quantitative data, such as the spatiotemporal distribution of GFP-tagged proteins in signaling pathways. The data acquired are, however, usually a composition of both the GFP-tagged proteins of interest and of an autofluorescent background, which both undergo photobleaching during imaging. We here present a mathematical model based on ordinary differential equations that successfully describes the shuttling of intracellular Mig1-GFP under changing environmental conditions regarding glucose concentration. Our analysis separates the different bleaching rates of Mig1-GFP and background, and the background-to-Mig1-GFP ratio. By applying our model to experimental data, we can thus extract the Mig1-GFP signal from the overall acquired signal and investigate the influence of kinase and phosphatase on Mig1. We found a stronger regulation of Mig1 through its kinase than through its phosphatase when controlled by the glucose concentration, with a constant (de)phosphorylation rate independent of the glucose concentration. By replacing the term for decreasing excited Mig1-GFP concentration with a constant, we were able to reconstruct the dynamics of Mig1-GFP, as it would occur without bleaching and background noise. Our model effectively demonstrates how data, acquired with an optical microscope, can be processed and used for a systems biology analysis of signal transduction pathways.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Sott, Kristin,1974Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)kristin
(författare)
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Smedh, Maria,1968Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg(Swepub:gu)xsmema
(författare)
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Millat, T
(författare)
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Dahl, Peter,1965Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,University of Gothenburg(Swepub:gu)xdapet
(författare)
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Wolkenhauer, O
(författare)
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Goksör, Mattias,1975Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg(Swepub:gu)xgokma
(författare)
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Göteborgs universitetInstitutionen för fysik (GU)
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:MOLECULAR BIOSYSTEMS: Royal Society of Chemistry (RSC)7:1, s. 215-2231742-206X1742-2051
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Frey, S
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Sott, Kristin, 1 ...
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Smedh, Maria, 19 ...
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Millat, T
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Dahl, Peter, 196 ...
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Wolkenhauer, O
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Goksör, Mattias, ...
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Göteborgs universitet
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Chalmers tekniska högskola