Sökning: onr:"swepub:oai:gup.ub.gu.se/171260" >
Poly(ADP-ribose) po...
Poly(ADP-ribose) polymerase cleavage during apoptosis: when and where?
-
Soldani, C (författare)
-
Lazzè, M C (författare)
-
Bottone, M G (författare)
-
visa fler...
-
- Tognon, Gianluca, 1976 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
-
Biggiogera, M (författare)
-
Pellicciari, C E (författare)
-
Scovassi, A I (författare)
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2001
- 2001
- Engelska.
-
Ingår i: Experimental cell research. - : Elsevier BV. - 0014-4827. ; 269:2, s. 193-201
- Relaterad länk:
-
https://gup.ub.gu.se...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Poly(ADP-ribose) polymerase-1 (PARP-1) plays the active role of "nick sensor" during DNA repair and apoptosis, when it synthesizes ADP-ribose from NAD(+) in the presence of DNA strand breaks. Moreover, PARP-1 becomes a target of apoptotic caspases, which originate two proteolytic fragments of 89 and 24 kDa. The precise relationship between PARP-1 activation and degradation during apoptosis is still a matter of debate. In human Hep-2 cells driven to apoptosis by actinomycin D, we have monitored PARP-1 activity by the mAb 10H, which is specific for the ADP-ribose polymers, and we have observed that poly(ADP-ribose) synthesis is a very early response to the apoptotic stimulus. The analysis of the presence and fate of the p89 proteolytic fragment revealed that PARP-1 proteolysis by caspases is concomitant with poly(ADP-ribose) synthesis and that p89 migrates from the nucleus into the cytoplasm in late apoptotic cells with advanced nuclear fragmentation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Apoptosis
- Blotting
- Western
- Cell Line
- Cell Nucleus
- metabolism
- Cytoplasm
- metabolism
- DNA Repair
- Dactinomycin
- pharmacology
- Enzyme Activation
- Flow Cytometry
- Fluorescein-5-isothiocyanate
- metabolism
- Humans
- Immunohistochemistry
- Indicators and Reagents
- pharmacology
- Microscopy
- Confocal
- Microscopy
- Fluorescence
- Phosphatidylserines
- chemistry
- Poly(ADP-ribose) Polymerases
- metabolism
- Propidium
- pharmacology
- Protein Binding
- Protein Synthesis Inhibitors
- pharmacology
- Time Factors
- Tumor Cells
- Cultured
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas