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Differential regula...
Differential regulation of mesothelial cell fibrinolysis by transforming growth factor beta 1.
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- Falk, Peter, 1962 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna,Institute of Surgical Sciences
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Ma, C (författare)
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Chegini, N (författare)
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visa fler...
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- Holmdahl, Lena, 1954 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
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visa färre...
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(creator_code:org_t)
- 2000
- 2000
- Engelska.
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Ingår i: Scandinavian journal of clinical and laboratory investigation. - 0036-5513. ; 60:6, s. 439-47
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Inflammation and tissue trauma during the surgical procedure reduce the peritoneal fibrinolytic capacity. These conditions promote adhesion formation, and are associated with increased expression of transforming growth factor beta 1 (TGF-beta1). The objective of the present study was to investigate whether TGF-beta1 regulates the expression of fibrinolytic components in peritoneal mesothelial cells. Human peritoneal mesothelial cells (HPMC) were cultured and treated with various concentrations of human recombinant TGF-beta1 (0.1, 1.0 and 10 ng/mL) for 24 h. Levels of tissue- and urokinase plasminogen activator (t-PA and uPA), plasminogen activator inhibitor type-1 (PAI-1) and type-2 (PAI-2) mRNA and protein were assessed by quantitative reverse transcriptase polymerase chain reaction (Q-RT-PCR) and ELISA, respectively. HPMC expressed these components at the gene and protein level. TGF-beta1 downregulated, dose-dependently t-PA mRNA and protein to about 50% of control values (p = 0.0010), and doubled PAI-1 protein production (p = 0.0008) compared to untreated controls. Although uPA gene expression increased in cells exposed to TGF-beta1, the corresponding protein concentration in conditioned media did not. PAI-2 was not affected, either at the gene or protein level. In conclusion, the results indicate that fibrinolytic capacity of mesothelial cells is reduced by TGF-beta1, suggesting that peritoneal adhesion formation induced by TGF-beta1 may be mediated, in part, through reduction in fibrin degradation capacity at an early stage of peritoneal tissue repair.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Enzyme-Linked Immunosorbent Assay
- Epithelial Cells
- drug effects
- Fibrinolysis
- genetics
- Gene Expression Profiling
- Humans
- Peritoneal Cavity
- pathology
- physiopathology
- Plasminogen Activator Inhibitor 1
- genetics
- Plasminogen Activator Inhibitor 2
- genetics
- RNA
- Messenger
- genetics
- metabolism
- Receptors
- Cell Surface
- genetics
- Receptors
- Urokinase Plasminogen Activator
- Recombinant Proteins
- pharmacology
- Reverse Transcriptase Polymerase Chain Reaction
- Tissue Plasminogen Activator
- genetics
- Transforming Growth Factor beta
- pharmacology
- Urokinase-Type Plasminogen Activator
- genetics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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