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Monitoring the osmo...
Monitoring the osmotic response of single yeast cells through force measurement in the environmental scanning electron microscope
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- Jansson, Anna, 1985 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Nafari, Alexandra, 1980 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Hedfalk, Kristina, 1969 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg,Göteborgs Universiet
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- Olsson, Eva, 1960 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Svensson, Krister, 1969- (författare)
- Karlstads universitet,Institutionen för ingenjörsvetenskap och fysik (from 2013),Materialfysik
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- Sanz-Velasco, Anke, 1971 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers Tekniska Högskola
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(creator_code:org_t)
- 2013-12-20
- 2014
- Engelska.
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Ingår i: Measurement science and technology. - : IOP Publishing. - 0957-0233 .- 1361-6501. ; 25:2
- Relaterad länk:
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http://dx.doi.org/10...
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http://iopscience.io...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://research.cha...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- We present a measurement system that combines an environmental scanning electron microscope (ESEM) and an atomic force microscope (AFM). This combination enables studies of static and dynamic mechanical properties of hydrated specimens, such as individual living cells. The integrated AFM sensor provides direct and continuous force measurement based on piezoresistive force transduction, allowing the recording of events in the millisecond range. The in situ ESEM-AFM setup was used to study Pichia pastoris wild-type yeast cells. For the first time, a quantified measure of the osmotic response of an individual yeast cell inside an ESEM is presented. With this technique, cell size changes due to humidity variations can be monitored with nanometre accuracy. In addition, mechanical properties were extracted from load-displacement curves. A Young's modulus of 13-15 MPa was obtained for the P. pastoris yeast cells. The developed method is highly interesting as a complementary tool for the screening of drugs directed towards cellular water transport activity and provides new possibilities of studying mechanosensitive regulation of aquaporins.
Ämnesord
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
Nyckelord
- atomic force microscopy
- cell viability
- environmental scanning electron microscopy
- in situ
- osmotic response
- single cell characterization
- yeast cell
- atomic force microscopy
- Physics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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