Sökning: onr:"swepub:oai:gup.ub.gu.se/209206" > Carbachol-induced M...
Fältnamn | Indikatorer | Metadata |
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000 | 05411naa a2200781 4500 | |
001 | oai:gup.ub.gu.se/209206 | |
003 | SwePub | |
008 | 240528s2013 | |||||||||||000 ||eng| | |
024 | 7 | a https://gup.ub.gu.se/publication/2092062 URI |
024 | 7 | a https://doi.org/10.1152/ajpcell.00141.20132 DOI |
040 | a (SwePub)gu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Pelaseyed, Thaher,d 1979u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xpelth |
245 | 1 0 | a Carbachol-induced MUC17 endocytosis is concomitant with NHE3 internalization and CFTR membrane recruitment in enterocytes. |
264 | 1 | b American Physiological Society,c 2013 |
520 | a We have reported that transmembrane mucin MUC17 binds PDZ protein PDZK1, which retains MUC17 apically in enterocytes. MUC17 and transmembrane mucins MUC3 and MUC12 are suggested to build the enterocyte apical glycocalyx. Carbachol (CCh) stimulation of the small intestine results in gel-forming mucin secretion from goblet cells, something that requires adjacent enterocytes to secrete chloride and bicarbonate for proper mucin formation. Surface labeling and confocal imaging demonstrated that apically expressed MUC17 in Caco-2 cells and Muc3(17) in murine enterocytes were endocytosed upon stimulation with CCh. Relocation of MUC17 in response to CCh was specific as MUC3 and MUC12 did not relocate following CCh stimulation. MUC17 colocalized with PDZK1 under basal conditions, while MUC17 relocated to the terminal web and into early endosomes after CCh stimulation. CCh stimulation concomitantly internalized the Na(+/)H(+) exchanger 3 (NHE3) and recruited cystic fibrosis transmembrane conductance regulator (CFTR) to the apical membranes, a process that was important for CFTR-mediated bicarbonate secretion necessary for proper gel-forming mucin unfolding. The reason for the specific internalization of MUC17 is not understood, but it could limit the diffusion barrier for ion secretion caused by the apical enterocyte glycocalyx or alternatively act to sample luminal bacteria. Our results reveal well-orchestrated mucus secretion and trafficking of ion channels and the MUC17 mucin. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Farmaceutiska vetenskaper0 (SwePub)301012 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Pharmaceutical Sciences0 (SwePub)301012 hsv//eng |
653 | a Animals | |
653 | a Biotinylation | |
653 | a Caco-2 Cells | |
653 | a Carbachol | |
653 | a pharmacology | |
653 | a Carrier Proteins | |
653 | a metabolism | |
653 | a Cell Membrane | |
653 | a drug effects | |
653 | a metabolism | |
653 | a Cholinergic Agonists | |
653 | a pharmacology | |
653 | a Cystic Fibrosis Transmembrane Conductance Regulator | |
653 | a drug effects | |
653 | a metabolism | |
653 | a Endocytosis | |
653 | a drug effects | |
653 | a Endosomes | |
653 | a drug effects | |
653 | a metabolism | |
653 | a Enterocytes | |
653 | a drug effects | |
653 | a metabolism | |
653 | a Humans | |
653 | a Male | |
653 | a Mice | |
653 | a Mice | |
653 | a Inbred C57BL | |
653 | a Microscopy | |
653 | a Confocal | |
653 | a Mucins | |
653 | a metabolism | |
653 | a Protein Transport | |
653 | a Sodium-Hydrogen Antiporter | |
653 | a metabolism | |
653 | a Time Factors | |
700 | 1 | a Gustafsson, Jenny K,d 1981u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xgjenv |
700 | 1 | a Gustafsson, Ida Ju Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk genetik och klinisk genetik,Institute of Biomedicine, Department of Medical and Clinical Genetics4 aut |
700 | 1 | a Ermund, Annau Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xermua |
700 | 1 | a Hansson, Gunnar C.,d 1951u Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology4 aut0 (Swepub:gu)xhagun |
710 | 2 | a Göteborgs universitetb Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi4 org |
773 | 0 | t American journal of physiology. Cell physiologyd : American Physiological Societyg 305:4q 305:4x 1522-1563x 0363-6143 |
856 | 4 | u https://europepmc.org/articles/pmc3891215 |
856 | 4 8 | u https://gup.ub.gu.se/publication/209206 |
856 | 4 8 | u https://doi.org/10.1152/ajpcell.00141.2013 |
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