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Chemical and lectin...
Chemical and lectin-gold electron microscopical studies of the expression of the Galalpha1-determinant in the pig aorta.
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- Hallberg, Eva C., 1966 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
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Strokan, V (författare)
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Cairns, T D (författare)
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visa fler...
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- Breimer, Michael, 1951 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
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Samuelsson, B E (författare)
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visa färre...
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(creator_code:org_t)
- 1998
- 1998
- Engelska.
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Ingår i: Xenotransplantation. - 0908-665X. ; 5:4, s. 246-56
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- In the xenotransplantation research field, pig aortic endothelial cells are frequently used in different model systems, e.g., for the study of the xenoantibody-antigen reaction. The Gal(alpha1),3Gal determinant is the major target for human xenoreactive antibodies in pig tissue. Characterisation of the Gal(alpha1)- distribution in pig aortic endothelial cells is thus important for understanding the reaction occurring at the endothelial cells during the xenorejection. We have determined the complete structure of the major Gal(alpha1),3Gal terminated glycolipid, Gal(alpha1),3nLc4Cer. Structural studies were performed on isolated glycosphingolipids by mass spectrometry and NMR spectroscopy. The results show a predominance of the pentasaccharide among the Gal(alpha1)-terminated glycolipids but also the presence of several Gal(alpha1)-terminated glycolipids with extended carbohydrate core chains. Ultrastructural localisation of the Galalpha1-antigen in pig aorta was done by lectin-gold electron microscopic studies of aortic wall sections using the Griffonia simplicifolia isolectin B4. Gal(alpha1)-determinants are predominantly localised on the luminal surface of pig aortic endothelial cells and endothelial cells of vasa vasorum and, to a lesser extent, vascular subendothelium.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Nyckelord
- Animals
- Aorta
- Carbohydrate Sequence
- Disaccharides
- analysis
- Endothelium
- Vascular
- chemistry
- ultrastructure
- Galactosides
- analysis
- Glycolipids
- analysis
- chemistry
- Glycosphingolipids
- analysis
- chemistry
- Gold
- Humans
- Lectins
- Magnetic Resonance Spectroscopy
- Mass Spectrometry
- Microscopy
- Electron
- Microscopy
- Immunoelectron
- Molecular Sequence Data
- Swine
- Transplantation
- Heterologous
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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