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Rapid 3D fluorescen...
Rapid 3D fluorescence imaging of individual optically trapped living immune cells
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Wolfson, D. (författare)
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Steck, M. (författare)
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- Persson, Martin, 1979 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)
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McNerney, G. (författare)
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Popovich, A. (författare)
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- Goksör, Mattias, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)
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Huser, T. (författare)
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(creator_code:org_t)
- 2014-01-13
- 2015
- Engelska.
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Ingår i: Journal of Biophotonics. - : Wiley. - 1864-0648 .- 1864-063X. ; 8:3, s. 208-216
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We demonstrate an approach to rapidly characterize living suspension cells in 4 dimensions while they are immobilized and manipulated within optical traps. A single, high numerical aperture objective lens is used to separate the imaging plane from the trapping plane. This facilitates full control over the position and orientation of multiple trapped cells using a spatial light modulator, including directed motion and object rotation, while also allowing rapid 4D imaging. This system is particularly useful in the handling and investigation of the behavior of non-adherent immune cells. We demonstrate these capabilities by imaging and manipulating living, fluorescently stained Jurkat T cells.
Ämnesord
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
Nyckelord
- 3-D imaging
- Confocal microscopy
- Fluorescence microscopy
- Jurkat cells
- Optical tweezers
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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