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Membrane engineerin...
Membrane engineering for reduced acetic acid stress: insights from Zygosaccharomyces bailii
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- Lindahl, Lina, 1984 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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Santos, Aline X S (författare)
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Genheden, Samuel (författare)
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- Eriksson, Leif A, 1964 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
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Riezman, Howard (författare)
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- Olsson, Lisbeth, 1963 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Bettiga, Maurizio, 1978 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2015
- 2015
- Engelska.
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Ingår i: Oral presentation at 12th Yeast Lipid Conference, May 20-22 2015, Ghent, Belgium.
- Relaterad länk:
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Abstract
Ämnesord
Stäng
- The high concentration of acetic acid released during pretreatment of lignocellulose raw material is a major obstacle to the microbial production of bio-based products. Acetic acid enters the cell mainly by passive diffusion across the plasma membrane and inhibits yeast by mechanisms such as reduction of intracellular pH, accumulation of the acetate anion, and by signaling effects triggering cell death. Through extensive characterization of the acetic acid tolerant yeast Zygosaccharomyces bailii, we have identified the cell membrane as a target for strain engineering with potential to increase acetic acid tolerance in Saccharomyces cerevisiae. We propose membrane permeability as a key component for Z. bailii’s acetic acid tolerance. We have previously shown that Z. bailii has a unique ability to remodel its plasma membrane upon acetic acid stress, to strongly increase its fraction of complex sphingolipids, at the expense of a drastic reduction of glycerophospholipids1. Here we further demonstrate the involvement of complex sphingolipids in acetic acid tolerance by decreasing sphingolipid synthesis using the drug myriocin, and characterize the acetic acid tolerance in terms of growth and intracellular pH. Furthermore we show the impact of complex sphingolipids on membrane physical properties using in silico membrane simulations. Ongoing membrane engineering of S. cerevisiae can potentially give additional strength to our findings. References 1 Lindberg et al. (2013), Lipidomic Profiling of Saccharomyces cerevisiae and Zygosaccharomyces bailii Reveals Critical Changes in Lipid Composition in Response to Acetic Acid Stress, PLoS One 8: e73936.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- acetic acid
- tolerance
- sphingolipids
- molecular dynamic simulations
- tolerance
Publikations- och innehållstyp
- vet (ämneskategori)
- kon (ämneskategori)