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Noninvasive imaging...
Noninvasive imaging of radiolabeled exosome-mimetic nanovesicle using Tc-99m-HMPAO
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Hwang, D. W. (författare)
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Choi, H. (författare)
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- Jang, Su Chul, 1984 (författare)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre
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Yoo, M. Y. (författare)
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Park, J. Y. (författare)
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Choi, N. E. (författare)
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Oh, H. J. (författare)
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Ha, S. (författare)
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Lee, Y. S. (författare)
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Jeong, J. M. (författare)
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Gho, Yong Song, 1964 (författare)
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Lee, D. S. (författare)
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(creator_code:org_t)
- 2015-10-26
- 2015
- Engelska.
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 5
- Relaterad länk:
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https://www.nature.c...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Exosomes known as nano-sized extracellular vesicles attracted recent interests due to their potential usefulness in drug delivery. Amid remarkable advances in biomedical applications of exosomes, it is crucial to understand in vivo distribution and behavior of exosomes. Here, we developed a simple method for radiolabeling of macrophage-derived exosome-mimetic nanovesicles (ENVs) with Tc-99m-HMPAO under physiologic conditions and monitored in vivo distribution of Tc-99m-HMPAO-ENVs using SPECT/CT in living mice. ENVs were produced from the mouse RAW264.7 macrophage cell line and labeled with Tc-99m-HMPAO for 1 hr incubation, followed by removal of free Tc-99m-HMPAO. SPECT/CT images were serially acquired after intravenous injection to BALB/c mouse. When ENVs were labeled with Tc-99m-HMPAO, the radiochemical purity of Tc-99m-HMPAO-ENVs was higher than 90% and the expression of exosome specific protein (CD63) did not change in Tc-99m-HMPAO-ENVs. Tc-99m-HMPAOENVs showed high serum stability (90%) which was similar to that in phosphate buffered saline until 5 hr. SPECT/CT images of the mice injected with Tc-99m-HMPAO-ENVs exhibited higher uptake in liver and no uptake in brain, whereas mice injected with Tc-99m-HMPAO showed high brain uptake until 5 hr. Our noninvasive imaging of radiolabeled-ENVs promises better understanding of the in vivo behavior of exosomes for upcoming biomedical application.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- IN-VIVO
- DRUG-DELIVERY
- INTRAVENOUS-INJECTION
- CELLS
- BIODISTRIBUTION
- LEUKOCYTES
- INFECTION
- BRAIN
- HMPAO
- VITRO
- Multidisciplinary Sciences
- ATES OF AMERICA
- V107
- P6328
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Hwang, D. W.
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Choi, H.
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Jang, Su Chul, 1 ...
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Yoo, M. Y.
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Park, J. Y.
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Choi, N. E.
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visa fler...
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Oh, H. J.
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Ha, S.
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Lee, Y. S.
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Jeong, J. M.
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Gho, Yong Song, ...
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Lee, D. S.
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
- Artiklar i publikationen
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Scientific Repor ...
- Av lärosätet
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Göteborgs universitet