Fluorescence enhancement from single DNA molecules confined in SiO 2 nanochannels

• We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

Ämnesord

NATURVETENSKAP  -- Fysik (hsv//swe)

NATURAL SCIENCES  -- Physical Sciences (hsv//eng)

NATURVETENSKAP  -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)

NATURAL SCIENCES  -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)

Nyckelord

DNA

Fluorescence microscopy

Nanofluidics

Single molecule

Emission enhancement

Emission intensity

Enhancement effects

Fluorescence enhancement

Optical phenomena

Quantitative fluorescence microscopy

Single DNA molecules

Molecules

Emission enhancement

Publikations- och innehållstyp

ref (ämneskategori)

kon (ämneskategori)