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Pregnancy-Associate...
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Adamczyk, Barbara,1985Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
(författare)
Pregnancy-Associated Changes of IgG and Serum N-Glycosylation in Camel (Camelus dromedarius).
- Artikel/kapitelEngelska2016
Förlag, utgivningsår, omfång ...
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2016-08-03
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American Chemical Society (ACS),2016
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LIBRIS-ID:oai:gup.ub.gu.se/242326
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https://gup.ub.gu.se/publication/242326URI
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https://doi.org/10.1021/acs.jproteome.6b00439DOI
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Ämneskategori:art swepub-publicationtype
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The dromedary camel (Camelus dromedarius) is an agriculturally important species of high economic value but of low reproductive efficiency. Serum and IgG N-glycosylation are affected by physiological and pathogenic changes and might therefore be a useful diagnostic tool in camel livestock management. This study presents the first comprehensive annotation of the N-glycome from dromedary camel serum as well as their single-domain and conventional antibodies and its subsequent application for camel pregnancy diagnostics. N-glycans were released by PNGaseF, labeled with 2-aminobenzamide (2-AB), and analyzed by hydrophilic interaction liquid chromatography with fluorescent detection (HILIC-UPLC-FLD), enzymatic sequencing and mass spectrometry (MS). The use of a high-throughput robotic platform for sample preparation allowed the rapid generation of glycomics data from pregnant (n = 8) and nonpregnant (n = 8) camels of the Majaheem and Wadha breed. IgG N-glycans dominate the glycan profile of camel serum and present a mixture of core-fucosylated and noncore-fucosylated N-glycans which can contain N-glycolylneuraminic and N-acetylneuraminic acid. Significant pregnancy-associated but breed-independent increases in galactosylation, core-fucosylation, sialylation, and decreases in serum O-acetylation were observed. The monitoring of IgG and serum N-glycosylation presents an attractive complementary test for camel pregnancy diagnostics and presents an interesting tool for biomarker discovery in camel health and breeding.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Albrecht, Simone
(författare)
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Stöckmann, Henning
(författare)
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Ghoneim, Ibrahim M
(författare)
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Al-Eknah, Marzook
(författare)
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Al-Busadah, Khalid A S
(författare)
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Karlsson, Niclas G.,1966Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xkanic
(författare)
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Carrington, Stephen D
(författare)
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Rudd, Pauline M
(författare)
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Göteborgs universitetInstitutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of proteome research: American Chemical Society (ACS)15:9, s. 3255-651535-39071535-3893
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