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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004687naa a2200565 4500
001oai:gup.ub.gu.se/250460
003SwePub
008240910s2016 | |||||||||||000 ||eng|
009oai:research.chalmers.se:f706da2d-0ab1-4516-8e18-74a7abd96e9d
0247 a https://gup.ub.gu.se/publication/2504602 URI
0247 a https://doi.org/10.1039/c6fd00102e2 DOI
0247 a https://research.chalmers.se/publication/2476312 URI
040 a (SwePub)gud (SwePub)cth
041 a eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Lovric, Jelena,d 1980u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)lovric
2451 0a On the mechanism of electrochemical vesicle cytometry: chromaffin cell vesicles and liposomes
264 c 2016
264 1b Royal Society of Chemistry (RSC),c 2016
520 a The mechanism of mammalian vesicle rupture onto the surface of a polarized carbon fiber microelectrode during electrochemical vesicle cytometry is investigated. It appears that following adsorption to the surface of the polarized electrode, electroporation leads to the formation of a pore at the interface between a vesicle and the electrode and this is shown to be potential dependent. The chemical cargo is then released through this pore to be oxidized at the electrode surface. This makes it possible to quantify the contents as it restricts diffusion away from the electrode and coulometric oxidation takes place. Using a bottom up approach, lipid-only transmitter-loaded liposomes were used to mimic native vesicles and the rupture events occurred much faster in comparison with native vesicles. Liposomes with added peptide in the membrane result in rupture events with a lower duration than that of liposomes and faster in comparison to native vesicles. Diffusional models have been developed and suggest that the trend in pore size is dependent on soft nanoparticle size and diffusion of the content in the nanometer vesicle. In addition, it appears that proteins form a barrier for the membrane to reach the electrode and need to move out of the way to allow close contact and electroporation. The protein dense core in vesicles matrixes is also important in the dynamics of the events in that it significantly slows diffusion through the vesicle.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
650 7a NATURVETENSKAPx Kemix Analytisk kemi0 (SwePub)104012 hsv//swe
650 7a NATURAL SCIENCESx Chemical Sciencesx Analytical Chemistry0 (SwePub)104012 hsv//eng
653 a lipid-bilayer formation
653 a fusion pore
653 a amperometric measurements
653 a catecholamine content
653 a vesicular exocytosis
653 a secretory vesicles
653 a extended kiss
653 a full fusion
653 a release
653 a membrane
653 a Chemistry
7001 a Najafinobar, Neda,d 1985u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)nobar
7001 a Dunevall, Johan,d 1984u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)dunevall
7001 a Majdi, Soodabeh,d 1980u Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)soodabeh
7001 a Svir, I.u Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif4 aut
7001 a Oleinick, A.u Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif4 aut
7001 a Amatore, C.u Processus d'Activation Selective Par Transfert d'Energie Uni-Electronique ou Radiatif4 aut
7001 a Ewing, Andrew G,d 1957u Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)andrewe
7102 a Chalmers tekniska högskolab Institutionen för kemi och molekylärbiologi4 org
7730 t Faraday Discussionsd : Royal Society of Chemistry (RSC)g 193, s. 65-79q 193<65-79x 1359-6640x 1364-5498
8564 u http://dx.doi.org/10.1039/c6fd00102ey FULLTEXT
8564 8u https://gup.ub.gu.se/publication/250460
8564 8u https://doi.org/10.1039/c6fd00102e
8564 8u https://research.chalmers.se/publication/247631

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