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A novel AP2/ERF fam...
A novel AP2/ERF family transcription factor from Glycine soja, GsERF71, is a DNA binding protein that positively regulates alkaline stress tolerance in Arabidopsis
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Yu, Y. (författare)
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Duan, X. B. (författare)
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Ding, X. D. (författare)
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visa fler...
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Chen, C. (författare)
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Zhu, D. (författare)
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Yin, K. D. (författare)
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Cao, L. (författare)
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Song, X. W. (författare)
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Zhu, P. H. (författare)
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Li, Q. (författare)
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Nisa, Z. U. (författare)
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Yu, J. Y. (författare)
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Du, J. Y. (författare)
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Song, Y. (författare)
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Li, H. Q. (författare)
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- Liu, Beidong, 1972 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Zhu, Y. M. (författare)
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(creator_code:org_t)
- 2017-07-05
- 2017
- Engelska.
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Ingår i: Plant Molecular Biology. - : Springer Science and Business Media LLC. - 0167-4412 .- 1573-5028. ; 94:4-5, s. 509-530
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Alkaline soils are widely distributed all over the world and greatly limit plant growth and development. In our previous transcriptome analyses, we have identified several ERF (ethylene-responsive factor) genes that responded strongly to bicarbonate stress in the roots of wild soybean G07256 (Glycine soja). In this study, we cloned and functionally characterized one of the genes, GsERF71. When expressed in epidermal cells of onion, GsERF71 localized to the nucleus. It can activate the reporters in yeast cells, and the C-terminus of 170 amino acids is essential for its transactivation activity. Yeast one-hybrid and EMSA assays indicated that GsERF71 specifically binds to the cis-acting elements of the GCC-box, suggesting that GsERF71 may participate in the regulation of transcription of the relevant biotic and abiotic stress-related genes. Furthermore, transgenic Arabidopsis plants overexpressing GsERF71 showed significantly higher tolerance to bicarbonate stress generated by NaHCO3 or KHCO3 than the wild type (WT) plants, i.e., the transgenic plants had greener leaves, longer roots, higher total chlorophyll contents and lower MDA contents. qRT-PCR and rhizosphere acidification assays indicated that the expression level and activity of H+-ATPase (AHA2) were enhanced in the transgenic plants under alkaline stress. Further analysis indicated that the expression of auxin biosynthetic genes and IAA contents were altered to a lower extent in the roots of transgenic plants than WT plants under alkaline stress in a short-term. Together, our data suggest that GsERF71 enhances the tolerance to alkaline stress by up-regulating the expression levels of H+-ATPase and by modifying auxin accumulation in transgenic plants.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Bicarbonate stress
- ERF transcriptional factor
- Wild soybean
- Arabidopsis
- H+-ATPase
- Auxin
- ethylene-responsive factor
- genome-wide analysis
- membrane h+-atpase
- erf gene family
- abiotic stress
- transgenic tobacco
- osmotic-stress
- salt-stress
- molecular characterization
- root-growth
- Biochemistry & Molecular Biology
- Plant Sciences
- aney rl
- 1992
- journal of plant nutrition6th international symp on iron
- jul 14-19
- 1991
- logan
- ut
- v15
- p2063
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Yu, Y.
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Duan, X. B.
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Ding, X. D.
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Chen, C.
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Zhu, D.
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Yin, K. D.
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visa fler...
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Cao, L.
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Song, X. W.
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Zhu, P. H.
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Li, Q.
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Nisa, Z. U.
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Yu, J. Y.
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Du, J. Y.
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Song, Y.
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Li, H. Q.
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Liu, Beidong, 19 ...
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Zhu, Y. M.
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
- Artiklar i publikationen
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Plant Molecular ...
- Av lärosätet
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Göteborgs universitet