Mycophenolate mofetil ameliorates perivascular T lymphocyte inflammation and reduces the double-negative T cell population in SLE-prone MRLlpr/lpr mice.

• Effects on T lymphocyte mediated pathology, phenotypes, and functions in MRLlpr/lpr mice given mycophenolate mofetil (MMF) (100 mg/kg/day) via drinking water or controls given ip cyclophosphamide (CYC) injections (1.8 mg/mouse/week) or water were described. Both MMF and CYC treatment diminished kidney and large salivary gland perivascular cell infiltrates, reduced profoundly double-negative (DN) T cell frequencies, decreased total lymphocyte number in spleen, and increased in vitro proliferative response to Con A. IFN-gamma and IL-10 in supernatants from Con A stimulated spleen cells were augmented after MMF but not CYC treatment. MMF treatment increased whereas CYC reduced IL-12 in serum. Kidney expressions of IFN-gamma, IL-10, and IL-12 mRNA were unaffected by MMF but decreased by CYC. Our results demonstrate that MMF and CYC suppress perivascular T lymphocyte inflammation by reducing the DN T cell population and by amelioration of T cell function. The varying cytokine patterns suggest different mechanisms of the two drugs.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine (hsv//eng)

Nyckelord

Animals

Anti-Inflammatory Agents

Non-Steroidal

pharmacology

B-Lymphocytes

drug effects

immunology

Cell Count

Cell Division

Concanavalin A

pharmacology

Cyclophosphamide

pharmacology

Cytokines

blood

genetics

Disease Models

Animal

Female

Hypersensitivity

Delayed

Immunophenotyping

Immunosuppressive Agents

pharmacology

Kidney

blood supply

cytology

immunology

Lupus Erythematosus

Systemic

immunology

Male

Mice

Mice

Inbred MRL lpr

Mycophenolic Acid

analogs & derivatives

pharmacology

RNA

Messenger

Salivary Glands

blood supply

cytology

Spleen

cytology

immunology

T-Lymphocytes

cytology

drug effects

immunology

Vasculitis

pathology

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)