Sökning: onr:"swepub:oai:gup.ub.gu.se/261814" >
Detection of post-t...
Detection of post-translational modifications using solid-phase proximity ligation assay.
-
- de Oliveira, Felipe Marques Souza (författare)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab
-
- Mereiter, Stefan (författare)
- i3S – Instituto de Investigação e Inovação em Saúde and IPATIMUP – Institute of Molecular Pathology and Immunology of the University of Porto, Portugal
-
- Lönn, Peter (författare)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab
-
visa fler...
-
- Siart, Benjamin (författare)
- Department of Anthropology, University of Vienna, Austria; Department of Behavioral Biology, University of Vienna, Austria
-
- Shen, Qiujin (författare)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab
-
- Heldin, Johan (författare)
- Uppsala universitet,Molekylära verktyg,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
-
- Raykova, Doroteya, 1986- (författare)
- Uppsala universitet,Molekylära verktyg,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
-
- Karlsson, Niclas G., 1966 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,Department of Medical Biochemistry and Cell Biology at Institute of Biomedicine, Gothenburg University, Sweden
-
- Polom, Karol (författare)
- Department of Surgical Oncology, Medical University of Gdansk, Poland; General Surgery and Surgical Oncology Department, Università deli Studi di Siena, Italy.
-
- Roviello, Franco (författare)
- General Surgery and Surgical Oncology Department, Università deli Studi di Siena, Italy.
-
- Reis, Celso A (författare)
- ) i3S – Instituto de Investigação e Inovação em Saúde and IPATIMUP – Institute of Molecular Pathology and Immunology of the University of Porto, Portugal; Instituto de Ciências Biomédicas Abel Salazar (ICBAS), University of Porto, Portugal; Faculty of Medicine of the University of Porto, Portugal
-
- Kamali-Moghaddam, Masood (författare)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2018
- 2018
- Engelska.
-
Ingår i: New biotechnology. - : Elsevier BV. - 1876-4347 .- 1871-6784. ; 45:October, s. 51-59
- Relaterad länk:
-
https://gup.ub.gu.se...
-
visa fler...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Post-translational modifications (PTMs) regulate protein activities to help orchestrate and fine-tune cellular processes. Dysregulation of PTMs is often related with disorders and malignancies, and may serve as a precise biomarker of disease. Developing sensitive tools to measure and monitor low-abundant PTMs in tissue lysates or serum will be instrumental for opening up new PTM-based diagnostic avenues. Here, we investigate the use of solid-phase proximity ligation assay (SP-PLA) for detection of different PTMs. The assay depends on the recognition of the target protein molecule and its modification by three affinity binders. Using antibodies and lectins, we applied the method for detection of glycosylated CD44 and E-Cadherin, and phosphorylated p53 and EGFR. The assay was found to have superior dynamic range and limit of detection compared to standard ELISAs. In summary, we have established the use of SP-PLA as an appropriate method for sensitive detection of PTMs in lysates and sera, which may provide a basis for future PTM-based diagnostic and prognostic biomarkers.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
de Oliveira, Fel ...
-
Mereiter, Stefan
-
Lönn, Peter
-
Siart, Benjamin
-
Shen, Qiujin
-
Heldin, Johan
-
visa fler...
-
Raykova, Dorotey ...
-
Karlsson, Niclas ...
-
Polom, Karol
-
Roviello, Franco
-
Reis, Celso A
-
Kamali-Moghaddam ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinsk biotek ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinsk biotek ...
-
och Medicinsk biotek ...
- Artiklar i publikationen
-
New biotechnolog ...
- Av lärosätet
-
Göteborgs universitet
-
Uppsala universitet